We narrowed to 24,628 results for: Nes

1. pCLC-mEos2

   Plasmid

   #38010

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Use

   Tags

   mEos2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR221-Firefly

   Plasmid

   #79495

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Firefly Luciferase
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 7iBi89 (rat fibronectin)

   Plasmid

   #14065

   Depositor

   Richard Hynes

   Article

   Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

   Insert

   Rat Fibronectin 7iBi89 (Fn1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Insert was cut out of pGEM3 with SalI and SacI an…

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

4. CeNL(Ca2+)_19μ-pcDNA3

   Plasmid

   #111927

   Purpose

   Cyan color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_19μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E67D, F92W, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMaCTag-P17

   Plasmid

   #120028

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mEos4b
   

   Use

   Pcr template

   Tags

   mEos4b

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMaCTag-Z06

   Plasmid

   #120049

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   sfGFP
   

   Use

   Pcr template

   Tags

   sfGFP

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. K560CLM T324C

   Plasmid

   #24460

   Depositor

   Ron Vale

   Article

   Yildiz et al Science. 2004 Jan 30. 303(5658):676-8.

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S T324C/C421A

   Promoter

   Available Since

   Aug. 12, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pENTR221-sGFP

   Plasmid

   #79508

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   superGFP
   

   Use

   Multisite gateway entry clone

   Tags

   6xHis

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC-1-AtU6-Y

   Plasmid

   #197779

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   AtU6 promoter for sgRNA assembly
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC-1-OsU6-X

    Plasmid

    #197780

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    OsU6 promoter for sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p1DT (chicken beta 1 integrin)

    Plasmid

    #14066

    Depositor

    Richard Hynes

    Article

    Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

    Insert

    Chicken Beta 1 Integrin (ITGB1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pYFP (GB0053)

    Plasmid

    #68197

    Purpose

    Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    YFP coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaCTag-P01

    Plasmid

    #120012

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxBFP
    

    Use

    Pcr template

    Tags

    moxBFP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Nano-lantern(Ca2+)_117/pcDNA3

    Plasmid

    #51985

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-2GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pMC-1-CmYLCV-6

    Plasmid

    #197699

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    CmYLCV promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMC-6-RLuc-9

    Plasmid

    #197739

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Renilla luciferase (tomato optimized)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGB3_omega2

    Plasmid

    #68239

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLIK TT 3xFLAG Luciferase neo

    Plasmid

    #98392

    Purpose

    Lentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-G6-mCherry

    Plasmid

    #66533

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter G6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - G6

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMaCTag-P32

    Plasmid

    #120043

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    S-Tag
    

    Use

    Pcr template

    Tags

    S-Tag

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX330A_D10A-1x3

    Plasmid

    #58773

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMaCTag-Z25

    Plasmid

    #120068

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMC-1-CmYLCV-4

    Plasmid

    #197694

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    CmYLCV promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLIK 3XFLAG-MKK4-EE-NLS hygro

    Plasmid

    #87779

    Purpose

    Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

27. pMaCTag-P08

    Plasmid

    #120019

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTRANS_263

    Plasmid

    #91127

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Nano-lantern(Ca2+)_600/pRSETB

    Plasmid

    #51976

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pMaCTag-Z03

    Plasmid

    #120046

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxCerulean3
    

    Use

    Pcr template

    Tags

    moxCerulean3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGE-attBCC-FRT-mW (pTL788)

    Plasmid

    #115159

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-CC FRT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX302-PTP-SL-V5 puro

    Plasmid

    #87774

    Purpose

    Expresses V5 tagged human PTP-SL

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    PTP-SL (PTPRR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Khc73-GFP

    Plasmid

    #31746

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Khc73 (Khc-73 Fly)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Metallothionein

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pMA5.1 (mouse integrin alpha 5 probe)

    Plasmid

    #14097

    Depositor

    Richard Hynes

    Article

    Yang et al Development. 1993 Dec . 119(4):1093-105.

    Insert

    Mouse Alpha 5 Integrin (Itga5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

35. Nano-lantern-Actin/pEGFP-C1

    Plasmid

    #51972

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pMaCTag-Z14

    Plasmid

    #120057

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeptune2.5
    

    Use

    Pcr template

    Tags

    mNeptune2.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pETM6-H9-mCherry

    Plasmid

    #66532

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H9

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H9

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMaCTag-Z16

    Plasmid

    #120059

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Dronpa3
    

    Use

    Pcr template

    Tags

    Dronpa3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Nano-lantern-peroxisome/pcDNA3

    Plasmid

    #51974

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    SKL

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

40. Nano-lantern(Ca2+)_300/pRSETB

    Plasmid

    #51977

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only