We narrowed to 23,171 results for: crispr

1. pICSL1P5_crU626

   Plasmid

   #191780

   Purpose

   Cas12a guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC1819_pCR8-DR-SMN2-2-DR

   Plasmid

   #118653

   Purpose

   Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   DR-SMN2-2-DR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCSh3-mRFP

   Plasmid

   #170983

   Purpose

   Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   mRFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSHS248

   Plasmid

   #87354

   Purpose

   S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S/S867C/N1054C

   Promoter

   T7

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dSp-VP64-RTA

   Plasmid

   #99675

   Purpose

   Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Tags

   VP64-RTA

   Expression

   Mammalian

   Mutation

   dead Cas9

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pICSL1P3_AtU626Ter26

   Plasmid

   #191771

   Purpose

   Cas9 guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   SpCas9 guide scaffold with AtU626 promoter/terminator
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pICSL11197

   Plasmid

   #191784

   Purpose

   SpCas9 expression in plants

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   AtUbi10::Cas9 +13 introns-T-E9
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC1820_pCR8-DR-RG6-SA-DR

    Plasmid

    #118654

    Purpose

    Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    DR-RG6-SA-DR
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cas-Acr (insertion 5)

    Plasmid

    #128116

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (insertion 5)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cas-Acr (N39A)

    Plasmid

    #128117

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (N39A)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMLS234

    Plasmid

    #73682

    Purpose

    SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. NAX_CAGGS_CAS9_VPR_EYFP

    Plasmid

    #167890

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJEC578

    Plasmid

    #174366

    Purpose

    dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    dCas9 fused to SYNZIP18
    

    Expression

    Bacterial

    Promoter

    J23150

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW212

    Plasmid

    #113234

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->CCATC

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFETCh_SIX4_iso1

    Plasmid

    #128238

    Purpose

    Donor vector for 3' FLAG tag of human SIX4_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SIX4_iso1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEc-crRNA1

    Plasmid

    #170088

    Purpose

    encodes E. coli type I-E single spacer array

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E single spacer array
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    J23119

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OA-1050S (UASt-dCasRx)

    Plasmid

    #132419

    Purpose

    Express dCasRx under UASt promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    CasRx
    UASt+hsp70
    

    Expression

    Insect

    Promoter

    UASt

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cas-Acr (wtAcrIIA4)

    Plasmid

    #128115

    Purpose

    Expresses SpyCas9 fused to wild-type AcrIIA4

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (wtAcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBC2102-reci

    Plasmid

    #117171

    Purpose

    U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_wtCas9 without NLS
    

    Use

    Lentiviral

    Promoter

    U6 Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cas-Acr (D14A/G38A)

    Plasmid

    #128118

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (D14A/G38A)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCUX1.1.0-gDNA

    Plasmid

    #112434

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1

    Depositor

    Kevin White

    Insert

    CUX1 (CUX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. dSp-Rta 125-190

    Plasmid

    #99664

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated RTA activation domain ( 125-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. L1p6_barley_sgRNA_scaffold

    Plasmid

    #231033

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. L1p5_barley_sgRNA_scaffold

    Plasmid

    #231032

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. L1p4_barley_sgRNA_scaffold

    Plasmid

    #231031

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. L1p3_barley_sgRNA_scaffold

    Plasmid

    #231030

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmirGLO-mir302-8mer4x (pEZ185)

    Plasmid

    #229059

    Purpose

    Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-302a

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    Human miR-302a binding sites
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmirGLO-mir1-8mer4x (pEZ184)

    Plasmid

    #229058

    Purpose

    Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-1

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    Human miR-1 binding sites
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRC065d

    Plasmid

    #225979

    Purpose

    dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)

    Depositor

    Jesse Zalatan

    Article

    Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

    Inserts

    dRxCas13d
    buj1 crRNA (non-cognate DR) targets the unstructured RBS
    

    Use

    CRISPR

    Promoter

    J23107 and J23119

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMRS-GFP

    Plasmid

    #220197

    Purpose

    mini-Tn7 GFP expression vector

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    promoterless

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. dSp-Rta 125-175

    Plasmid

    #99668

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated RTA activation domain (125-175)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. dSp-VP64-RTA(75-190)

    Plasmid

    #99669

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    VP64-RTA(75-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. dSp-p65 (1-100)

    Plasmid

    #99660

    Purpose

    Expresses dSp Cas9 fused to truncated p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated p65 activation domain (1-100)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. dSp-p65 (50-150)

    Plasmid

    #99661

    Purpose

    Expresses dSp Cas9 fused to truncated p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated p65 activation domain (50-150)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. dSp-Rta 75-190

    Plasmid

    #99663

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated RTA activation domain (75-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. dSp-Rta 75-175

    Plasmid

    #99666

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated RTA activation domain (75-175)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. dSp-p65 Full (1-261)

    Plasmid

    #99654

    Purpose

    Expresses dSp Cas9 fused to p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    p65 activation domain

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. dSp-p65 (150-261)

    Plasmid

    #99655

    Purpose

    Expresses dSp Cas9 fused to truncated p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated p65 activation domain (150-261)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. dSp-p65 (100-261)

    Plasmid

    #99656

    Purpose

    Expresses dSp Cas9 fused to truncated p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated p65 activation domain (100-261)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. dSp-p65 (200-261)

    Plasmid

    #99657

    Purpose

    Expresses dSp Cas9 fused to truncated p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated p65 activation domain (200-261)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. dSp-p65 (1-200)

    Plasmid

    #99658

    Purpose

    Expresses dSp Cas9 fused to truncated p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated p65 activation domain (1-200)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. dSp-p65 (1-150)

    Plasmid

    #99659

    Purpose

    Expresses dSp Cas9 fused to truncated p65 activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated p65 activation domain (1-150)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA)

    Plasmid

    #196827

    Purpose

    Express dpdpCas13b with AAAA mutation

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

    Plasmid

    #196828

    Purpose

    Express dpdpCas13b with AAAA mutation

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGTag Hygro BFP nLAP +0

    Plasmid

    #194317

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pUCSh2-mRFP

    Plasmid

    #170982

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGTag Hygro EGFP nLAP -2

    Plasmid

    #194322

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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