We narrowed to 6,296 results for: pcDNA 3.1

Showing: 4601 - 4650 of 6296 results

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myr-palm_mCherry2-mEGFP

Plasmid

#127816

Purpose

Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry2-mEGFP

Expression

Mammalian

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-mito-CitroffRH

Plasmid

#158548

Purpose

Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitroffRH

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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TR therapy reporter

Plasmid

#179083

Purpose

Fluorescent reporter for alternative splicing

Depositor

Thurman Wheeler

Article

Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

Inserts

DsRed1
EGFP

Expression

Mammalian

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p176 Grg-1s Myc His

Plasmid

#11065

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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EGFP-PH

Plasmid

#96948

Purpose

EGFP-PLC pleckstrin homology domain for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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LacI INCENP

Plasmid

#45236

Purpose

Target INCENP to lac operator sequence

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

LacI, INCENP-Δcen, mCherry (lacI )

Tags

LacI (lac repressor protein) and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

t7

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFH2.20_mCherry-MS2x4

Plasmid

#205535

Purpose

Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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miniCMV-iRFP670-tDeg

Plasmid

#185403

Purpose

iRFP670-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

iRFP670-tDeg

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm_mCherry-mEGFP

Plasmid

#127815

Purpose

Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry-mEGFP

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Bongwoori-R3

Plasmid

#101658

Purpose

A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-R3

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pZZMP154

Plasmid

#231760

Purpose

Mammalian expression of LZ-SPO11

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

LZ-SPO11 (Spo11 Budding Yeast, Mouse)

Tags

Flag-LeucineZipper

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBT140

Plasmid

#27493

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

Nuclear localization signal-Cre

Tags

nuclear localization signal

Expression

Mammalian

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS20

Plasmid

#48342

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS20

Tags

TN-XXL

Expression

Mammalian

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
TRPML2-HA

Plasmid

#18828

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
Rtn3c-mEmerald

Plasmid

#186963

Purpose

Express Rtn3c with C-terminus mEmerald

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

RTN3C (Rtn3 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEQ1007

Plasmid

#83938

Purpose

express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Article

Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

Insert

IRS1

Tags

6xHIS

Expression

Mammalian

Mutation

R833L in IRS1 (please see depositor comment)

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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Venus-KRasCT

Plasmid

#99016

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Venus KRasCT

Expression

Mammalian

Promoter

CM

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.21_mCherry-MS2x6

Plasmid

#205536

Purpose

Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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BLING 1

Plasmid

#171647

Purpose

Expresses bioluminescent glutamate indicator in mammalian cells

Depositor

Assaf Gilad

Article

Petersen et al ACS Synth Biol. 2023 Jul 14. doi: 10.102

Insert

BLING 1

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBBI

Plasmid

#65795

Purpose

Vector for iCre mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al Transgenic Res. 2013 Aug;22(4):873-5. do

Insert

iCre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDD2701

Plasmid

#175078

Purpose

Mammalian expression of Flag-tagged KSHV ORF45

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8 )

Expression

Mammalian

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFH2.11_Gag-MCP_ZF2-P2A-eGFP

Plasmid

#205526

Purpose

Express HIV Gag-MCP_ZF2-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP_ZF2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Ectodomain M400C

Plasmid

#159642

Purpose

Contains integrin alpha v residues 1–960 with the M400C mutation

Depositor

Timothy Springer

Article

Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

Integrin alpha v ectodomain

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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mGarnet2-RITA

Plasmid

#104314

Purpose

codes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

mGarnet2-RITA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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psgRNA-ccdB

Plasmid

#99915

Purpose

Basic cloning vector for the expression of imaging-based optimization sgRNA

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Cenp-B-targeted Aurora B FRET sensor

Plasmid

#45232

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CENP-B targeted Aurora B FRET sensor

Expression

Mammalian

Promoter

t7

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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H2B-mGarnet

Plasmid

#104311

Purpose

codes for a fusion of fluorescent protein mGarnet and human histon 2B (H2B), makes the H2B visible in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

H2B-mGarnet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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PH-dL5**

Plasmid

#96946

Purpose

PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

dL5 and mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID

Plasmid

#188653

Purpose

Expresses SaAID in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nSaCas9-AID-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

pCMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform III

Plasmid

#120319

Purpose

Septin 11 isoform III mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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TRPML2-MYC

Plasmid

#18829

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
CENP-B mCherry PP1 gamma

Plasmid

#45221

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

CENP-B mCherry PP1 gamma

Expression

Mammalian

Promoter

T7

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II 15

Plasmid

#35177

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains the C-terminal repeats 16-52

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
Aahn

Plasmid

#112158

Purpose

A genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.

Depositor

Bradley Baker

Article

Sepehri Rad et al Sci Rep. 2018 May 2;8(1):6911. doi: 10.1

Insert

Aahn

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb2E7

Plasmid

#184681

Purpose

NIR-Fb to HIV gp41

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb2E7

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPML3-CFP

Plasmid

#18834

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

CFP

Expression

Mammalian

Mutation

Q165R, F210Y

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-AAVS1-sg

Plasmid

#194716

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-Pol-II CC

Plasmid

#35180

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains C-terminal domain repeats (1-22)X2

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
NbALFA-TEV219

Plasmid

#201487

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbALFA-TEV219

Tags

TEV219

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPML3-MYC

Plasmid

#18833

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
nonphosphorylatable Mis12-targeted Aurora B FRET sensor

Plasmid

#45229

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

non phosphorylatable Mis12-targeted Aurora B FRET sensor

Expression

Mammalian

Mutation

T-A mutation in substrate sequence

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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NbV5-TEV219

Plasmid

#201481

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-TEV219

Tags

TEV219

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45222

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

C-term Hec1-targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
SuperNova-TGON3

Plasmid

#131859

Purpose

Expresses NuperNova fused to trans-Golgi protein TGON3

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

TGON3 (rat)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-Pol-II DC

Plasmid

#35181

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal domain repeats 1-22 and 31-52

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDWUER

Plasmid

#37094

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

WU polyomavirus early region (WUPyV_gp4, WUPyV_gp5 WU polyomavirus)

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TNXXL-CTS68

Plasmid

#48343

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS68

Tags

TN-XXL

Expression

Mammalian

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
mGarnet-RITA

Plasmid

#104313

Purpose

codes for a fusion of fluorescent protein mGarnet and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

mGarnet-RITA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH234-Tier1-PmPGK1-tTA

Plasmid

#169574

Purpose

Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 6,296 results for: pcDNA 3.1