Addgene

Showing: 1841 - 1860 of 11666 results

Results per page:

Sort results by:

pcDNA5-FRT-TO-Nsp13_GC3opt

Plasmid

#157714

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1 3xFlag IFIT3

Plasmid

#53553

Purpose

mammalian expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CSF1R-V5/HIS

Plasmid

#201984

Purpose

expression of human CSF1R receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

CSF1R receptor tyrosine kinase (CSF1R Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAintron-CDC50A-c3xFLAG

Plasmid

#215702

Purpose

Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CDC50A (TMEM30A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-myc3-L11

Plasmid

#20936

Depositor

Yue Xiong

Article

Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

Insert

Ribosomal protein L11 (RPL11 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-RPS6 (pDD1902)

Plasmid

#52913

Purpose

contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

Depositor

Dirk Dittmer

Article

Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

Insert

HAS6 (RPS6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-20nm-EBFP2

Plasmid

#231185

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nm-EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_LAMP1_HaloTag9

Plasmid

#175541

Purpose

Lyzosomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_V5-APEX2-HMOX1

Plasmid

#85824

Purpose

Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-HMOX1 (HMOX1 Human, Synthetic)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mCherry-Rheb

Plasmid

#192436

Purpose

Encodes mCherry-tagged wild-type Rheb.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb (RHEB Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hOlig1-myc/pCDNA3.1

Plasmid

#197337

Purpose

Mammalian expression of an epitope-tagged hOLIG1

Depositor

Charles Stiles

Insert

OLIG1(pSM1-3) (OLIG1 Human)

Use

Tags

Myc-his

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLIPglu-600u

Plasmid

#13572

Depositor

Wolf Frommer

Article

Fehr et al J Biol Chem. 2003 May 23. 278(21):19127-

Insert

FLIPglu F16A

Use

Tags

ECFP and EYFP

Expression

Mammalian

Mutation

F16A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-TEK-V5/HIS

Plasmid

#205611

Purpose

Expression of human TEK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TEK receptor tyrosine kinase (TEK Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc3-Cul7

Plasmid

#20695

Depositor

Yue Xiong

Article

Andrews et al Oncogene. 2006 Aug 3. 25(33):4534-48.

Insert

cullin 7 (CUL7 Human)

Use

Tags

Myc3

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TfR-OVA

Plasmid

#64600

Purpose

Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

Depositor

Martin Zenke, Sandra Diebold

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Use

Tags

Transmembrane domain of Transferrin Receptor (TfR…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-NP-V5-B/BRISBANE/60/2008

Plasmid

#187923

Purpose

Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Kristijan Ramadan, Ivana Marinovic-Terzic

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Use

Tags

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 CLN3-GFP

Plasmid

#78110

Purpose

Mammalian expression of human CLN3 with C terminal GFP fusion

Depositor

Thomas Braulke

Insert

CLN3 (CLN3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only