161,727 results

1. pCMV-PE6d

   Plasmid

   #207854

   Purpose

   Mammalian expression of PE6d prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6d
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

   Plasmid

   #199371

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-CMV-Galpha-s ONE-GO

   Plasmid

   #189731

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-s ONE-GO
   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. PB-TO-NFIA.1-SOX9

   Plasmid

   #182310

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NFIA-T2A-SOX9 (NFIA, SOX9 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Piggybac

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-MMLV_RT (mut H)

   Plasmid

   #165546

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

   Plasmid

   #55639

   Purpose

   Flp-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-flag YAP2 5SA

   Plasmid

   #27371

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP2 (YAP1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Serines at positions 61, 109, 127, 128, 131, 163,…

   Promoter

   Available since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGfaABC1D-nLac

   Plasmid

   #53131

   Purpose

   expression of lacZ driven by human GfaABC1D promoter

   Depositor

   Michael Brenner

   Article

   Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

   Insert

   Gfa2 (GFAP Human)
   

   Use

   Mouse Targeting

   Tags

   LacZ, NLS, and mP1

   Expression

   Mammalian

   Mutation

   contains ABC1D regions of Gfa2 promoter, -1757 to…

   Promoter

   human Gfa2

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-HA-rat Nrxn2beta AS4(-)

    Plasmid

    #58268

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn2beta AS4(-) (Nrxn2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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