163,606 results

1. EIF2AK3 gRNA (BRDN0001145848)

   Plasmid

   #77166

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK3 (EIF2AK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL50004

   Plasmid

   #50316

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, mCherry variant of RFP (Discosoma sp.)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

   Plasmid

   #205281

   Purpose

   CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.

   Depositor

   Matthias Prigge

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a Gx-NL

   Plasmid

   #169105

   Purpose

   Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKSJ155

   Plasmid

   #27126

   Depositor

   Alan Finkelstein

   Article

   Kienker et al J Gen Physiol. 2000 Oct . 116(4):587-98.

   Insert

   Colicin Ia + Ia immunity protein
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pJK225

   Plasmid

   #71124

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC16A9

   Plasmid

   #131876

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC16A9 (SLC16A9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pFA6a-hphMX6-Purg1-3FLAG

   Plasmid

   #19356

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   urg1 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

   Plasmid

   #70191

   Purpose

   Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   ClpX (clpX )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. EIF2AK3 gRNA (BRDN0001146328)

    Plasmid

    #77167

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge