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pAAV-CamKIIa-moxBFP-IRES-Flpo

Plasmid

#194977

Purpose

AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

moxBFP, Flpo

Use

AAV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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AL12R-HSP70

Plasmid

#161010

Purpose

PP7-tagged reporter driven by the Arabidopsis HSP70 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-CMV-GFP

Plasmid

#133017

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under CMV promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Carl-ReNL-KDEL_pcDNA3

Plasmid

#89535

Purpose

Red color variant of bright luminescent protein, targeted to the endoplasmic reticulum

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

Calreticulin and KDEL

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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AY42_pS.Donor.R5

Plasmid

#100291

Purpose

CCR5-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE M33 flag

Plasmid

#1952

Depositor

Robert Kingston

Article

Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

Insert

M33 (Cbx2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pRS-puro shNEDD4L #2

Plasmid

#27017

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #2 (NEDD4L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-L2T3D

Plasmid

#33277

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L2 (L2 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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dFP-mini

Plasmid

#120543

Purpose

Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein mini (dFP-mini)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TN-XXL pcDNA3

Plasmid

#45797

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

ECFP and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pDrop

Plasmid

#184872

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Carl-YeNL-KDEL_pcDNA3

Plasmid

#89516

Purpose

Yellow color variant of bright luminescent protein, targeted to the endoplasmic reticulum

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

Calreticulin and KDEL

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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HA-mEOS3.2-TRF2 HR Donor

Plasmid

#86845

Purpose

HR Donor for N-terminal HA-mEOS3.2-TRF2 fusion at endogenous TRF2 locus in human cells.

Depositor

Thomas Cech

Article

Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

Insert

HA-mEOS3.2-TRF2 at endogenous locus (TERF2 Human)

Tags

HA-mEOS3.2

Expression

Mammalian

Promoter

Endogenous TRF2 promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_150/pcDNA3

Plasmid

#51984

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2G

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(cAMP-3.3)/pcDNA3

Plasmid

#53595

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Expression

Mammalian

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pT7-L1T1L

Plasmid

#33286

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L1 (L1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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Carl-CeNL-KDEL_pcDNA3

Plasmid

#89509

Purpose

Cyan color variant of bright luminescent protein, targeted to the endoplasmic reticulum

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

Calreticulin and KDEL

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_11/pcDNA3

Plasmid

#51987

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-4GS

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
mPlum-CENPB-N-22

Plasmid

#55957

Purpose

Localization: Nucleus/Centromeres, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mPlum

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(cAMP-0.4)/pcDNA3

Plasmid

#53593

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Expression

Mammalian

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-Flag-HA-Merlin

Plasmid

#27104

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Tags

Flag and HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-GFP-PTEN-WT

Plasmid

#30391

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

GFP-PTEN-WT (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-WT

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
JH04

Plasmid

#73513

Purpose

mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mRuby2::PH::tbb-2

Tags

mRuby2

Promoter

mex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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LAMP-OeNL_pcDNA3

Plasmid

#89525

Purpose

Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-S4T3D

Plasmid

#33285

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S4 (S4 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pVimentin-LSSmOrange

Plasmid

#37134

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytochrome C oxidase subunit VIII and human…

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-TM

Plasmid

#176886

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

Ig kappa-FRISZ-PDGFR TM

Use

AAV

Promoter

hSyn

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-M1T1L

Plasmid

#33289

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M1 (M1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_peroxisome_C1

Plasmid

#85064

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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