We narrowed to 21,801 results for: Sis
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Plasmid#124854PurposeMutagenesis of Gria2DepositorInsertGria2 (Gria2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2
Plasmid#176252PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by fullDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-ccvTIR1-NOSt
Plasmid#108546PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGExpressionMutationChanged F79 to GPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria1
Plasmid#124867PurposeMutagenesis of Gria1DepositorInsertGria1 (Gria1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMNZ-neo-Flag-TNFR1 L380A
Plasmid#43949DepositorInsertTumor Necrosis Factor Receptor 1 (TNFRSF1A Human)
UseTagsFlag*ExpressionMutationL380APromoterAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorInsertHero11 (C19orf53 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria2
Plasmid#124868PurposeMutagenesis of Gria2DepositorInsertGria2 (Gria2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-4eCOL2A1
Plasmid#97211PurposeFor cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectorsDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseVector is designed for cloning and generation of …TagsExpressionMutationPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-mRuby2
Plasmid#100730PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and mRuby2ExpressionMammalianMutationLong isoformPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAX-2
Plasmid#210130Purposeknock out BAX in mammalian cellsDepositorInsertApoptosis regulator BAX (BAX Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGria3
Plasmid#124855PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin1
Plasmid#124865PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)
Plasmid#100742PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD8A (CD8A Human)
UseTagsLOVpep and tagRFP657ExpressionMammalianMutationTruncatedPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-KATNA1
Plasmid#180650PurposeLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.DepositorInsertKATNA1 (KATNA1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GGACAGCACUCCCUUGAAAPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)
Plasmid#153442PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.DepositorInsertRc.XDocB
UseTags3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero13
Plasmid#187929PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero13DepositorInsertHero13 (BEX3 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303A
Plasmid#118363PurposeThis plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK1 C121S
Plasmid#53165PurposeExpresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationC121SPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TAP(FLAG-HA) CYLD
Plasmid#60031PurposeCYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintainedDepositorInsertCylindromatosis (CYLD Human)
UseRetroviralTagsFLAG, HA, and IRES PuroExpressionMammalianMutationPromoterLTR-driven expressionAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD8-dCherry-FRB
Plasmid#100739PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)DepositorInsertCD8A (CD8A Human)
UseTagsFRB and dCherryExpressionMammalianMutationDark mCherry (K70N) non-fluorescentPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-NGFR:LMP1-IRES-mRFP
Plasmid#36974DepositorUseRetroviralTagsExpressionMammalianMutationNGFR:LMP1 chimeric receptor consists of aa 1–276 …PromoterCMVAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-2xFLAG
Plasmid#130979PurposeExpression of KIF1C-2xFLAG in mammalian cells.DepositorInsertKIF1C-2xFLAG (KIF1C Human)
UseTags2xFLAGExpressionMammalianMutation4 silent mutations that make this construct resis…PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria3
Plasmid#124869PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-tetO-Mek1-shRNA
Plasmid#53179PurposeExpresses shRNA against mouse Mek1 from puromycin resistance retroviral vectorDepositorInsertMEK1 (Map2k1 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-TIR1-NOSt
Plasmid#108545PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGExpressionMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16-HSF1Δ∼PRD
Plasmid#71727Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RDDepositorInsertGal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Human, Budding Yeast, Herpes Simplex Virus protein VP16)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_E307K
Plasmid#234560PurposeCTNNA1 butterfly eye mutantDepositorInsertmEGFP:hCTNNA1_E307K (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationE307KPromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_R551A
Plasmid#234558PurposeCTNNA1 M2-M3 salt-bridge disrupting mutantDepositorInsertmEGFP:hCTNNA1_R551A (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationR551APromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM2,3-domain
Plasmid#234556PurposeCTNNA1 M2,3-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM2,3-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa 397-509PromoterCMVAvailable sinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM1-domain
Plasmid#234555PurposeCTNNA1 M1-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM1-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa 271-396PromoterCMVAvailable sinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtmiR173aTS-B/c
Plasmid#227964PurposeEntry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.DepositorInsertsB/c
AtmiR173aTS
UseTagsExpressionMutationPromoterNoneAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
MT2
Plasmid#223330PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)
UseTags3HA-TurboID-EGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HA-CBS(opt)-FLAG
Plasmid#230088Purposeexpressing human CBSDepositorInsertCBS (CBS Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationMultiple wobble mutationsPromoterCMVAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HA-L2HGDH(opt)-FLAG
Plasmid#230089Purposeexpressing human L2HGDHDepositorInsertL2HGDH (L2HGDH Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationMultiple wobble mutationsPromoterCMVAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MT3
Plasmid#223331PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
UseTags3HA-EGFP-miniTurboIDExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MT4
Plasmid#223332PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
UseTags3HA-miniTurboID-EGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuper Retro GFP DGK zeta human
Plasmid#224579PurposeTo stably downregulate the expression of human DGK zeta in human cellsDepositorInsertsh RNAi Diacylglycerol kinase zeta human (GTPBP6 Human)
UseRNAi and RetroviralTagsExpressionMutationPromoterH1Available sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUSSTOP-Δα3
Plasmid#202056PurposeE. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.DepositorInsertAtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
UseTags6xHis and GFP11ExpressionBacterialMutationthird helix of homeodomain deleted (AA82-102)PromoterT7 PromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only