We narrowed to 5,263 results for: 290

1. pLN431 (FuGW-G8p-mKate2-1Pe )

   Plasmid

   #105186

   Purpose

   Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G8p-mKate2-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSK247

   Plasmid

   #131148

   Purpose

   dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   pSUP35, min pCYC1

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-SRAP

   Plasmid

   #84524

   Purpose

   Expresses murine SRAP domain (250 aa) in bacteria

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   SRAP domain (Hmces Mouse)
   

   Tags

   6xHis and S-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGR-Dx

   Plasmid

   #133408

   Purpose

   overexpression of a mutant human glucocorticoid receptor in mammalian cells

   Depositor

   Andrew Cato

   Article

   Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

   Insert

   NR3C1 (NR3C1 Human)
   

   Expression

   Mammalian

   Mutation

   N454D, A458T, R460D, D462C

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC44

   Plasmid

   #104818

   Purpose

   CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.04g057400
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSK226

   Plasmid

   #129268

   Purpose

   dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Rnq1
   

   Expression

   Yeast

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUPER.retro.puro-HCF-1-D

   Plasmid

   #36997

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   HCF-1 shRNA (HCFC1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 21, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

   Plasmid

   #105182

   Purpose

   Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(E2F1)p-ECFP (Cerulean) sponge control
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUPER.retro.puro-HCF-1-B

   Plasmid

   #36996

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Insert

   HCF-1 shRNA (HCFC1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pPN022

    Plasmid

    #91668

    Purpose

    Express sgRNA targeting human SHANK3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SHANK3 (SHANK3 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-SpyTag002 DA-MBP

    Plasmid

    #102832

    Purpose

    Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002 DA-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Reactive aspartic acid has been mutated to an ala…

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK RagA-RagC

    Plasmid

    #99649

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    10x His tag, Poly arginine linker, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

    Plasmid

    #159795

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPN021

    Plasmid

    #91667

    Purpose

    Express sgRNA targeting human SHANK3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SHANK3 (SHANK3 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5xtetO (tet3) Gene Desert

    Plasmid

    #131339

    Purpose

    gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Desert-gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA6/ERGIC2-WT

    Plasmid

    #51479

    Purpose

    Expresses ERGIC2-WT in mammalian cells

    Depositor

    Simon Kwok

    Article

    Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

    Insert

    ERGIC2-WT (ERGIC2 Human)
    

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. mCherry-SAC1-HLx8

    Plasmid

    #108139

    Purpose

    SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    

    Expression

    Mammalian

    Mutation

    SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETDuet RagA-RagC(L91P)

    Plasmid

    #99647

    Purpose

    Bacterial expression of RagA-RagC(L91P)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L91P

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JARID2-KO-gRNA-1

    Plasmid

    #131328

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

20. JARID2-KO-gRNA-2

    Plasmid

    #131327

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only