We narrowed to 48,849 results for: ang

1. p23-NES-RanCas13b-msfGFP-NES-Flag

   Plasmid

   #165073

   Purpose

   overexpression of RanCas13b in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   RanCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGES201

   Plasmid

   #190197

   Purpose

   For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. eiABE

   Plasmid

   #227144

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter

   Depositor

   Dali Li

   Article

   Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

   Insert

   bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
   

   Use

   Tags

   Expression

   Mutation

   D96R/E84R/V159R/H339A

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. eiCBE

   Plasmid

   #227148

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter

   Depositor

   Dali Li

   Article

   Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

   Insert

   bpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
   

   Use

   Tags

   Expression

   Mutation

   D96R/E84R/V159R/H339A

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. enIscB-T5E

   Plasmid

   #205411

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. miCBE

   Plasmid

   #205413

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Syn-ATP

   Plasmid

   #51819

   Purpose

   Optical reporter of presynaptic ATP

   Depositor

   Timothy Ryan

   Article

   Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

   Insert

   Chimeric Synaptophysin-mCherry-Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Within WT luciferase gene, changed Threonine 214 …

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EFS-foldon-GFP- PCP

   Plasmid

   #194503

   Purpose

   Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Insert

   foldon-GFP-PCP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α core promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

   Plasmid

   #71215

   Purpose

   dCas9 fused to EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-EGFP
   

   Use

   Lentiviral

   Tags

   dSpCas9-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCCL-PGK-SPdCas9-BFP-EGFP

    Plasmid

    #66821

    Purpose

    dCas9 fused to BFP and EGFP

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-EGFP
    

    Use

    Tags

    dSpCas9-BFP-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK-FLAG-TRAF4(K190R)

    Plasmid

    #58317

    Purpose

    FLAG-tagged mouse TRAF4 K190R mutant

    Depositor

    Ying Zhang

    Article

    Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

    Insert

    TNF receptor associated factor 4 (Traf4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K 190 to R

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-TRAF4(K190R)

    Plasmid

    #58319

    Purpose

    EGFP-tagged TRAF4 K190R mutant

    Depositor

    Ying Zhang

    Article

    Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

    Insert

    TNF receptor associated factor 4 (Traf4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K 190 to R

    Promoter

    CMV

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

14. TadA-8e-eIscBn-C-HMG-D

    Plasmid

    #227145

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
    

    Use

    Tags

    Expression

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eIscB-D

    Plasmid

    #227017

    Purpose

    Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    SV40 NLS-HMG-D-linker-OgeuIscB-v3-SV40-nucleoplasmin NLS
    

    Use

    Tags

    Expression

    Mutation

    D96R/E84R/V159R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hA3A*-IscBn

    Plasmid

    #227146

    Purpose

    Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
    

    Use

    Tags

    Expression

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TadA-8e-eIscBn-N-HMG-D

    Plasmid

    #227143

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter

    Depositor

    Dali Li

    Article

    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

    Insert

    bpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    

    Use

    Tags

    Expression

    Mutation

    D96R/E84R/V159R/H339A

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB-3xFlag-dCas13-MBD6MBD

    Plasmid

    #228233

    Purpose

    For targeted tethering of the MBD domain of human MBD6 using dCas13

    Depositor

    Chuan He

    Article

    Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

    Inserts

    dCas13
    MBD6 (MBD6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGMC00007

    Plasmid

    #166725

    Purpose

    non targeting sgRNA

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    Non-targeting guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLIC

    Plasmid

    #27989

    Depositor

    Zhihong Zhang

    Article

    Yang et al Biotechniques. 2010 Nov . 49(5):817-21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2011

    Availability

    Academic Institutions and Nonprofits only