Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,622 results for: pcas

Showing: 2821 - 2850 of 5622 results

  2. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

    Enlarge

  7. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

    Enlarge

  8. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

    Plasmid
    #208292
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpCas9(D10A)
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208292-plasmid-map-sequence-id-410847

    Enlarge

  14. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore
    Depositor
    Frank Bradke, Sebastián Dupraz
    Article
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

    Enlarge

  15. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid
    #101211
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101211-plasmid-map-sequence-id-198053

    Enlarge

  16. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid
    #101204
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101204-plasmid-map-sequence-id-198050

    Enlarge

  21. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

    Enlarge

  23. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid
    #234737
    Purpose
    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.
    Depositor
    Kirill Martemyanov
    Article
    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:
    Insert
    Nrl (Nrl Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234737-plasmid-map-sequence-id-466566

    Enlarge

  25. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid
    #229845
    Purpose
    A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Lamin nanobody
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    229845-plasmid-map-sequence-id-457771

    Enlarge

  27. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196878
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-N-gTBD
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196878-plasmid-map-sequence-id-408688

    Enlarge

  28. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

    Plasmid
    #196879
    Purpose
    Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196879-plasmid-map-sequence-id-408686

    Enlarge

  29. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

    Enlarge

  30. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196881
    Purpose
    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196881-plasmid-map-sequence-id-391885

    Enlarge

Showing: 2821 - 2850 of 5622 results