We narrowed to 4,333 results for: 1-Oct;
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Plasmid#21862DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStop codon for uORF1 converted to UCG (see author…PromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET14b-mEKLF
Plasmid#67834PurposeEncodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitutionDepositorInsertEKLF (Klf1 Mouse)
UseTagsExpressionBacterialMutationFull-length mEKLF is aa 20-376; amino acid 19 is …PromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA
Plasmid#173214PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
UseCRISPR; Prime editing (pe3)TagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9 (without sgRNA)
Plasmid#92353PurposeExpression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFlag-NLS-wild-type SpCas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCbhAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911(pL91)
Plasmid#124390PurposeE.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN aa1-125 (N125)
Plasmid#73378PurposeExpression of the N-terminal region of XPLN (amino acids 1–125, designated N125)DepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationExpresses N terminal amino acid 1-125 of XPLN (ar…PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACS_19
Plasmid#111651PurposeFunclib design ACS_19DepositorInsertACS_19
UseTags6xHisExpressionBacterialMutationPromoterpTacAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLX-MIFE-hM3Dq-PGK-mCherry
Plasmid#114294PurposeMifepristone-inducible expression of hM3Dq proteinDepositorInserthM3Dq
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ 3xFLAG-FEM1B WT
Plasmid#230993PurposeTransient overexpression of mouse FEM1B in mammalian cellsDepositorInsertFEM1B (Fem1b Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#173208PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I-TOSI donor
UseCRISPRTagsExpressionMammalianMutationPromoterhPGK1Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS exon37a pCAGGS
Plasmid#206086Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37aDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA pcDNA3
Plasmid#206053Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-BR1
Plasmid#192816PurposeExpresses BR1 in bacteriaDepositorInsertBR1 (BRI1 Mustard Weed)
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-MCRaeo-NG mcrBGA
Plasmid#192769PurposeExpresses NG-tagged MCRaeoBGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBCGA
Plasmid#192768PurposeExpresses NG-tagged MCRaeoBCGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBDGA
Plasmid#192767PurposeExpresses NG-tagged MCRaeoBDGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYI006
Plasmid#170840PurposeFRO6p::GUSPlus::tOCS in pFP100DepositorInsertFRO6p::GUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterFRO6Available sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV495
Plasmid#177702PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EBFP_Nick_Dual_sgRNA
Plasmid#178094PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EBFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EGFP_Nick_Dual_sgRNA
Plasmid#178095PurposeControl vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EGFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only