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pLCKOv3
Plasmid#209046PurposeLentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)
Plasmid#164193PurposeFreeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianMutationF391W-L398VPromoterSynAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12
Plasmid#101285PurposeLentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFPDepositorInsertE-Cadherin-GFP-H-Ras
UseLentiviralTagsGFPExpressionMammalianMutationH-Ras V12PromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(R306C)
Plasmid#110188PurposeExpression of mouse MeCP2 (R306C) in mammalian cellsDepositorInsertMeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationArg 306 CysPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(deltaNC)
Plasmid#110190PurposeExpression of mouse MeCP2 (deltaNC) in mammalian cellsDepositorInsertMeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationdelta N and C terminiPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCIG AKT3 K177M
Plasmid#73051PurposeHuman AKT3 K177MDepositorInsertAKT3 K177M (AKT3 Human)
UseTagsExpressionMammalianMutationK177MPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 S472A
Plasmid#73052PurposeHuman AKT3 S472ADepositorInsertAKT3 S472A (AKT3 Human)
UseTagsExpressionMammalianMutationS472APromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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miniSOG-Alpha-V-Integrin-25
Plasmid#57763PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertAlpha-V Integrin (ITGAV Human)
UseTagsminiSOGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCIG AKT3 S472E
Plasmid#73053PurposeHuman AKT3 S472EDepositorInsertAKT3 S472E (AKT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_01
Plasmid#158102Purpose5'UTR stem-loop luciferase reporter: 0kcal/molDepositorInsert5'UTR synthetic stem-loop 0kcal/mol
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N1_MeCP2(R111G)
Plasmid#110187PurposeExpression of mouse MeCP2 (R111G) in mammalian cellsDepositorInsertMeCP2_e2 FL [R111G] (mouse cDNA) (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationArg 111 GlyPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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