We narrowed to 59,329 results for: cloning

1. pTLNXA7

   Plasmid

   #49033

   Purpose

   FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Xenopus oocytes expression

   Tags

   chicken alpha7 signal sequence

   Promoter

   SP6

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. TAL-4GA1

   Plasmid

   #41504

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

   Insert

   4GA1
   

   Use

   Pcr cloning vector

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pGGEV_3'_Linker

   Plasmid

   #49289

   Purpose

   Golden Gate entry vector at position 3, small linker insert, final entry vector for the assembly

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. 9336-E27

   Plasmid

   #234310

   Purpose

   Gateway ORF Entry clone of human SMAD3 LSD-CSA with stop codon (for native or N-terminal fusions); Linker sites constitutively active and C-terminal sites dead

   Depositor

   Dominic Esposito

   Insert

   SMAD3 LSD-CSA (SMAD3 Human)
   

   Use

   Gateway entry clone

   Mutation

   T179D/S204D/S208D/S213D/S422A/S423A/S425A; Linker…

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. psLIB U6

   Plasmid

   #229956

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. psLIB EF1alpha

   Plasmid

   #229954

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. psLIB TRE

   Plasmid

   #229955

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVP293

   Plasmid

   #222062

   Purpose

   GreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

   Insert

   Filler
   

   Use

   Synthetic Biology; Greengate compatible cloning v…

   Mutation

   Backbone Esp3i sites have been domesticated

   Available Since

   Dec. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pVP294

   Plasmid

   #222063

   Purpose

   GreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

   Insert

   Filler
   

   Use

   Synthetic Biology; Greengate compatible cloning v…

   Mutation

   Backbone Esp3i sites have been domesticated

   Available Since

   Dec. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pVP096

    Plasmid

    #222043

    Purpose

    pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    mRFP1e
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pVP076

    Plasmid

    #222028

    Purpose

    Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    All Esp3i sites domesticated

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pVP077

    Plasmid

    #222029

    Purpose

    Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    All Esp3i sites domesticated

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. E2_pENTR_R4-R3_HSPter-RPS5A-NLS

    Plasmid

    #193371

    Purpose

    nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

    Depositor

    Shin-ichi Arimura

    Article

    Hosoda et al bioRxiv 2023.02.27.530312

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRB33

    Plasmid

    #165482

    Purpose

    pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Tags

    10xHis-FLAG-TEV

    Expression

    Mammalian

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFD-ePDZ-B1

    Plasmid

    #137113

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    ePDZ-B1
    

    Use

    Synthetic Biology

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFD-FN3

    Plasmid

    #137112

    Purpose

    iFLinkC Linker cloning

    Depositor

    Viktor Stein

    Article

    Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

    Insert

    FN3
    

    Use

    Synthetic Biology

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. VGF8a

    Plasmid

    #107572

    Purpose

    partial cDNA of a neurotrophin responsive gene

    Depositor

    Andrea Levi, Bruce Paterson

    Article

    Levi et al Science. 1985 Jul 26;229(4711):393-5.

    Insert

    VGF8a (Vgf Rat)
    

    Use

    Cloning vector

    Mutation

    partial cDNA (amino acid 65 - 617)

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGEV_2'_XcmI-LacZ

    Plasmid

    #49303

    Purpose

    Golden Gate entry vector at position 2, LacZ, final entry vector for the assembly

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGEV_3'_XcmI-LacZ

    Plasmid

    #49304

    Purpose

    Golden Gate entry vector at position 3, LacZ, final entry vector for the assembly

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGEV_5'_XcmI-LacZ

    Plasmid

    #49306

    Purpose

    Golden Gate entry vector at position 5, LacZ, final entry vector for the assembly

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge