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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,800 results for: emb

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Showing: 1881 - 1900 of 11800 results

  1. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  2. pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87260
    Purpose
    YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-AID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and AID
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87260-plasmid-map-sequence-id-176554

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  6. pHS_Nova

    Plasmid
    #239831
    Purpose
    NovaIscB mammalian expression
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NovaIscB
    Tags
    Nucleoplasmin NLS-3xHA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    CMV
    Available since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239831-plasmid-map-sequence-id-479113

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  10. AAV-GfaABC1D-lck-smV5-4x6T-WPRE

    Plasmid
    #196416
    Purpose
    Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Publication
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smV5
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CAG and GfaABC1D
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196416-plasmid-map-sequence-id-385161

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  14. TUBG1

    Plasmid
    #87954
    Purpose
    expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein
    Depositor
    Maria Alvarado-Kristensson
    Publication
    Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gamma-tubulin 1 (TUBG1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    whole protein, The C-terminal is a sh-gamma-tubul…
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    87954-plasmid-map-sequence-id-178205

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  15. pCS-H2B-EYFP

    Plasmid
    #53746
    Purpose
    contains fusion of human histone H2B to EYFP in pCS2+ vector.
    Depositor
    Sean Megason
    Publication
    Megason et al Methods Mol Biol. 2009;546:317-32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone H2B (H2BC21 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pGL2-hOct4 DE-SV40-Luc

    Plasmid
    #26279
    Purpose
    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.
    Depositor
    Rudolf Jaenisch
    Publication
    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1
    Insert
    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    See Depositor Comments below
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26279-plasmid-map-sequence-id-244682

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  20. C1-MPAct(delta1-6)-mRuby3

    Plasmid
    #155223
    Purpose
    F-actin bidning mutant of MPAct
    Depositor
    Tobias Meyer
    Publication
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Deletion of first 6 AA from F-tractin (9-14 from …
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155223-plasmid-map-sequence-id-310127

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Showing: 1881 - 1900 of 11800 results