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pKLV2-EF1adCas9VP64T2ABsd-W

Plasmid

#112919

Purpose

Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9VP64-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

Plasmid

#171129

Purpose

LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

U6 promoter entry plasmid for LwaCas13a crRNA cloning

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNRDc-V5

Plasmid

#51297

Purpose

expression of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
pYSD100

Plasmid

#212924

Purpose

Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SUC2 (SUC2 Budding Yeast)

Expression

Yeast

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pmIL-6 mut NF-kB

Plasmid

#61293

Purpose

drives luciferase from mouse IL-6 promoter with mutation in NF-kB site

Depositor

Gail Bishop

Article

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Insert

IL-6 promoter (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Mutated NF-kB binding site from GTGGGATTTTCCCA to…

Promoter

IL-6 promoter with mutant NF-kB binding site

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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SpyTag003-OspC

Plasmid

#159998

Purpose

Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag003-OspC (20-209) C129S

Tags

His6-tag and Thrombin cleavage site

Expression

Bacterial

Mutation

C129S mutation improves expression level.

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GST-MAGE-2-429

Plasmid

#89129

Purpose

bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pL0_AC-CEN_ARS_HIS

Plasmid

#202129

Purpose

Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CEN_ARS_HIS

Use

Synthetic Biology

Mutation

ARS sequence mutated to remove sapI site for dome…

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

Plasmid

#112921

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TMPRSS3

Plasmid

#221298

Purpose

Expression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TMPRSS3 (pLxIS) (Tmprss3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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Hoxb7 promoter

Plasmid

#21294

Depositor

Frank Costantini

Article

Srinivas et al Development. 1999 Apr . 126(7):1375-86.

Insert

Hoxb7 promoter (Hoxb7 Mouse)

Use

Promoter can be used to make transgenic mice

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3D21-1/3

Plasmid

#91296

Purpose

Protein expression and purification of human SH3 domain construct SH3D21-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D21-1/3 (SH3D21 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO15A-2/2

Plasmid

#91291

Purpose

Protein expression and purification of human SH3 domain construct MYO15A-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO15A-2/2 (MYO15A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPTAN1-1/1

Plasmid

#91292

Purpose

Protein expression and purification of human SH3 domain construct SPTAN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SPTAN1-1/1 (SPTAN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#184129

Purpose

For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1 (3kb)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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mr in pUASP

Plasmid

#112981

Purpose

P element vector for transposon insertion of mr (APC2) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kashevsky et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

mr (APC2) (mr Fly)

Use

P insertion vector

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
416-Met25 Kar2 printor HDEL

Plasmid

#32129

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

Printor (KLHL14 Human)

Tags

HDEL and Kar2

Expression

Yeast

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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