Addgene

We narrowed to 22,254 results for: Sele

Showing: 2881 - 2910 of 22254 results

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IRES-puro-3xFLAG

Plasmid

#204545

Purpose

Expresses 3xFLAG tag in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

3xFlag

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP

Plasmid

#204541

Purpose

Expresses GST-tagged MVP in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain (K81A)

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-Hs4EHP_52-234opt_AE

Plasmid

#148566

Purpose

Bacterial Expression of Hs4EHP_52-234opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt (EIF4E2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-52-GB1_AE

Plasmid

#148552

Purpose

Bacterial Expression of HsGIGYF1_33-52

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-52 (GIGYF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-71_AE

Plasmid

#148553

Purpose

Bacterial Expression of HsGIGYF1_33-71

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-71 (GIGYF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-71-GB1_AE

Plasmid

#148554

Purpose

Bacterial Expression of HsGIGYF1_33-71

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-71 (GIGYF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A_AE

Plasmid

#148555

Purpose

Bacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-103-E44AE45FQ87A (GIGYF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-103-GB1_AE

Plasmid

#148557

Purpose

Bacterial Expression of HsGIGYF1_33-103

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-103 (GIGYF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(553)-GFP

Plasmid

#197888

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_ER

Plasmid

#182815

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iFluoxSnFR_PM

Plasmid

#182816

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iFluoxSnFR_cyto

Plasmid

#182817

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_ER

Plasmid

#182818

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_PM

Plasmid

#182819

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_cyto

Plasmid

#182820

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iFluoxSnFR_ER

Plasmid

#182821

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iFluoxSnFR_PM

Plasmid

#182822

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iFluoxSnFR_cyto

Plasmid

#182823

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iEscSnFR_ER

Plasmid

#182812

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-CMV-RPS15A-3xHA plasmid

Plasmid

#192245

Purpose

overexpression vector of RPS15A-3XHA

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

RPS15A CDS without stop coden (RPS15A Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

Plasmid

#192230

Purpose

lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192229

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RPS15A sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192228

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2881 - 2910 of 22254 results

Results per page:

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