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Showing: 4801 - 4850 of 9723 results

  2. pFSynW SYT9 D197, 199, 330, 332N IRES GFP

    Plasmid
    #195703
    Purpose
    Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
    Depositor
    Edwin Chapman
    Article
    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.
    Insert
    Syt9 (Syt5 Mouse)
    Use
    Lentiviral
    Mutation
    full-length mouse SYT9 with D197, 199, 330, 332N …
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195703-plasmid-map-sequence-id-383663

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  3. Cav2.2 BBS W391A pcDNA3

    Plasmid
    #206077
    Purpose
    Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding
    Depositor
    Annette Dolphin
    Article
    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    W391A
    Promoter
    CMV
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206077-plasmid-map-sequence-id-406768

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  10. AAV1-DIO-eGFP-pvRPL10a

    Plasmid
    #202542
    Purpose
    Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.
    Depositor
    Zoe Donaldson
    Article
    Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75
    Insert
    RPL10a subunit of the ribosome
    Use
    AAV and Cre/Lox
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    optimized for prairie vole DNA sequence and optim…
    Promoter
    human synapsin
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    202542-plasmid-map-sequence-id-405350

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  14. pGADT7-δ

    Plasmid
    #197259
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-3 δ (AP3D1 Human)
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Val 549 Ile substitution; silent substitutions in…
    Promoter
    ADH1
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197259-plasmid-map-sequence-id-387016

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  18. pCapVQ329R

    Plasmid
    #183029
    Purpose
    the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase variant CapVQ329R
    Tags
    No
    Expression
    Bacterial
    Mutation
    glutamine 329 changed to arginine
    Promoter
    pBAD promoter
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183029-plasmid-map-sequence-id-361616

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  20. S+Q111R

    Plasmid
    #167173
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+Q111R
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167173-plasmid-map-sequence-id-334482

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  21. S+N122D

    Plasmid
    #167174
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+N122D
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed N at 111 to D
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167174-plasmid-map-sequence-id-334489

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  34. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid
    #154910
    Purpose
    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    Phytochrome B (PHYB Synthetic, Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB
    Promoter
    CMV
    Available Since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154910-plasmid-map-sequence-id-318440

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  38. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  39. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid
    #208043
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO2nc (SUMO2 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q90P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208043-plasmid-map-sequence-id-410267

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  41. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid
    #229498
    Purpose
    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.
    Depositor
    Davide Ruggero
    Article
    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/
    Insert
    MYC 5'Untranslated region + destabilized GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229498-plasmid-map-sequence-id-456853

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  43. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

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Showing: 4801 - 4850 of 9723 results