We narrowed to 13,948 results for: CRISPR-Cas9

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PX398 Nitratifractor salsuginis str DSM 16511 Cas9

Plasmid

#68334

Purpose

Nitratifractor salsuginis str DSM 16511 Cas9

Depositor

Feng Zhang

Insert

NsCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-ABE8e-dCas9-BSD

Plasmid

#226116

Purpose

Using promoter ef1a expresses ABE8e-dCas9 protein with Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-dCas9-EGFP-HaloTag

Plasmid

#169451

Purpose

expression of dCas9-EGFP-HaloTag

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9-EGFP-HaloTag

Use

Lentiviral

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

Plasmid

#107317

Purpose

Expresses SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9

Use

CRISPR

Tags

3xHA and 2xNLS and NLS

Expression

Mammalian

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109314

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

Plasmid

#140205

Purpose

Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Inserts

mCherry
sgRNA E1

Use

CRISPR and Synthetic Biology; Proof-of-concept te…

Mutation

G174D

Promoter

PelcA and U3

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

Plasmid

#47868

Purpose

This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

Plasmid

#165444

Purpose

Expresses ACX, rXRCC1 in mammalian cells

Depositor

Wei Leong Chew

Article

Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

Insert

APOBEC-nCas9-rXRCC1 (Apobec1 Streptococcus pyogenes, Synthetic, Rat)

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

Plasmid

#113693

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 gRNA Cassete

Use

AAV and CRISPR

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-Cas9-T2A-mCherry

Plasmid

#135012

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3x Flag, 3xFLAG (N terminal on insert), NLS, and …

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

Plasmid

#113685

Purpose

An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

inverted de-catalyzed SaCas9

Use

AAV, CRISPR, Cre/Lox, and Synthetic Biology

Tags

NLS and VP64

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

Plasmid

#112261

Purpose

Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

spCas9-p300 (EP300 Human, Synthetic)

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

Plasmid

#123611

Purpose

CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

nCas9-UGI-NLS-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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MMW2993 - human expression plasmid for SpCas9 Cluster 2

Plasmid

#101185

Purpose

Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

G582A/V583A/E584A/D585A/N588A

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

Plasmid

#120295

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgHtr2c (mouse/rat)

Plasmid

#249541

Purpose

Cre-dependent editing of Htr2c with SaCas9

Depositor

Larry Zweifel

Insert

Htr2c (Htr2c, Htr2c Mouse, Rat)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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