We narrowed to 14,467 results for: cas9 genes

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pYPQ-ABE-Cas9n-Act3.0

Plasmid

#178956

Purpose

It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA, mitfa:Cas9

Plasmid

#118844

Purpose

Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Expression

Bacterial

Promoter

gpdA;trpC

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSimpleII-NLS-NmCas9-HA-NLS(s)

Plasmid

#47867

Purpose

This plasmid encode NmCas9 with two NLS and a HA tag

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-dCas9-GFP

Plasmid

#64104

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9FL-P2A-turboGFP

Plasmid

#80941

Purpose

Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9FL

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416-Gal4-dCas9-VP64

Plasmid

#71128

Purpose

This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

Depositor

Ronald Davis

Article

Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

Insert

Gal4-dCas9-VP64

Tags

NLS n-terminal, N terminal Gal4 Activator domain,…

Expression

Yeast

Promoter

Tef1

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH-YE1-BE4max

Plasmid

#198549

Purpose

Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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HiFi-nCas9-NG_P2A-mCherry

Plasmid

#219936

Purpose

Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

HiFi-nCas9(D10A/R691A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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Dual-CRISPR-Cas9-U6-H1 (pBP48)

Plasmid

#218932

Purpose

The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

Depositor

Baoxu Pang

Article

Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

Type

Empty backbone

Use

CRISPR

Tags

2A-Puro and 3XHA

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-Cas9

Plasmid

#166601

Purpose

Encodes Cre-dependent Cas9 under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

Cas9

Use

AAV

Promoter

tetracycline response element

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-HF-nCas9

Plasmid

#163548

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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