We narrowed to 24,551 results for: p

1. pAAV-EF1a-CCreV

   Plasmid

   #140132

   Purpose

   can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Insert

   CCreV
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-NCreV

   Plasmid

   #140131

   Purpose

   can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Insert

   NCreV
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-DNMT3A-PuroR (ANV)

   Plasmid

   #71684

   Purpose

   Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-DNMT3A-PuroR_v2

   Plasmid

   #74407

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

   Plasmid

   #222853

   Purpose

   Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G, H435A, I253A, H310A

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A

   Plasmid

   #222855

   Purpose

   Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn binding

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G, H435A, I253A, H310A

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Phage-PGK-Flag-HA-FKBP-T(WT)

   Plasmid

   #181734

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Available Since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. HH02-LP Clone 2 heavy chain

   Plasmid

   #192175

   Purpose

   Clone 2 heavy chain (HH02)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 heavy chain (HH02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HK02-LP Clone 2 light chain

   Plasmid

   #192179

   Purpose

   Clone 2 light chain (HK02)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 light chain (HK02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid

    #224123

    Purpose

    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Q20A_Y23A_Y24F_F35A in La(SSB)1-194

    Promoter

    CMV

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti HsATP13A2 D508N

    Plasmid

    #171820

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D508N

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 (ATP13A2 Human)
    

    Use

    Lentiviral

    Mutation

    WT, D962N

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDule2-3-nitroTyrosine (A7)

    Plasmid

    #174079

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnCS_SEPT7_SEPT9_i5-TEV-Strep

    Plasmid

    #174502

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i5

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NINJA TC

    Plasmid

    #159274

    Purpose

    Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

    Depositor

    Nikhil Joshi

    Article

    Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    NINJA TC
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMVtight-HNF1A-FLAG-Hygro

    Plasmid

    #183232

    Purpose

    Lentiviral vector; Tet-on advanced system driving the expression of tagged HNF1A

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox A (HNF1A Human)
    

    Use

    Lentiviral

    Tags

    AviTag and FLAG tag

    Promoter

    tight TRE

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

    Plasmid

    #174498

    Purpose

    bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid

    #115694

    Purpose

    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Use

    CRISPR

    Tags

    NLS and NLS, HA

    Expression

    Insect and Mammalian

    Mutation

    None and fusion of repeat/tracrRNA to make a sgRNA

    Promoter

    EF-1 alpha and U6

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnCS_SEPT7_SEPT9_i3-TEV-Strep

    Plasmid

    #174501

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i3

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCD1C

    Plasmid

    #102954

    Purpose

    gene cloning of human CD1C

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

    Insert

    CD1C cDNA (CD1C Human)
    

    Use

    Cloning

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2-sgBAP1-1

    Plasmid

    #125837

    Purpose

    KO BAP1 gene

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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