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12,610 results

Showing: 1941 - 1960 of 12610 results
  1. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pLenti-mIFT88-EGFP

    Plasmid
    #149697
    Purpose
    Expresses mouse IFT88-EGFP in mammalian cells
    Depositor
    Insert
    IFT88 (Ift88 Mouse)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pINDUCER20_mStrawberry_Atg4BC74A

    Plasmid
    #161735
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy
    Depositor
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pAR18_StrepI_mFASm_H8_pET22b

    Plasmid
    #122847
    Purpose
    Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Insert
    mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pBS-AMVpl-δWT-nAchR

    Plasmid
    #97188
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Insert
    Delta nicotinic acetylcholine receptor (Chrnd Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1941 - 1960 of 12610 results