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px330-MRPsg2

Plasmid

#87190

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 2

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #2 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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B108-Otx2-IRES-Cre

Plasmid

#73993

Purpose

B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)

Expression

Mammalian

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

Plasmid

#133340

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P01

Plasmid

#120012

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z03

Plasmid

#120046

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GTU-g

Plasmid

#81094

Purpose

Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z14

Plasmid

#120057

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Pr-deCFP-MGapt

Plasmid

#67740

Purpose

OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deCFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAGM55361

Plasmid

#153227

Purpose

Control plasmid for knockout of AP3 gene

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for TRY and CPC and FAST marker

Use

Synthetic Biology

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GTH-g

Plasmid

#81104

Purpose

Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z16

Plasmid

#120059

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-18

Plasmid

#119997

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLIA CRX (CC#69)

Plasmid

#15128

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
Pr1-T7RNAP

Plasmid

#67738

Purpose

OR2-OR1-Pr1 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr1

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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