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Plasmid#106270PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasA
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCse2
Plasmid#106271PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasB
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas7
Plasmid#106272PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasC
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas5
Plasmid#106273PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasD
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRb delta CDK + S230
Plasmid#58907Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S230 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + T252
Plasmid#58910Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with T252 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
YAF2_pLX307
Plasmid#98383PurposeLentiviral expression of YAF2DepositorInsertYAF2 (YAF2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TLK1_pLX307
Plasmid#98378PurposeLentiviral expression of TLK1DepositorInsertTLK1 (TLK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_PIK3CA
Plasmid#45308DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_ARPM1
Plasmid#45259DepositorInsertARPM1 (ACTRT3 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWF073_LVt-EF1a-eGFP-ffLuc-PGK-BlastR
Plasmid#233447PurposeStable expression of eGFP-firefly luciferase (ffLuc)DepositorInserteGFP-firefly luciferase (ffLuc)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCR001H
Plasmid#233449PurposeExpresses ncHLAp-reactive T cell receptor (TCR001H)DepositorInsertTCR001H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCR004H
Plasmid#233450PurposeExpresses ncHLAp-reactive T cell receptor (TCR004H)DepositorInsertTCR004H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCR005H
Plasmid#233451PurposeExpresses ncHLAp-reactive T cell receptor (TCR005H)DepositorInsertTCR005H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCR008H
Plasmid#233452PurposeExpresses ncHLAp-reactive T cell receptor (TCR008H)DepositorInsertTCR008H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCR010H
Plasmid#233453PurposeExpresses ncHLAp-reactive T cell receptor (TCR010H)DepositorInsertTCR010H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCR012H
Plasmid#233454PurposeExpresses ncHLAp-reactive T cell receptor (TCR012H)DepositorInsertTCR012H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCR013H
Plasmid#233455PurposeExpresses ncHLAp-reactive T cell receptor (TCR013H)DepositorInsertTCR013H
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
PC1575
Plasmid#232088PurposeMammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.DepositorInsertuAb with cloning Esp3I restriction site
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-Scal-T2A-EGFP-Puro
Plasmid#234949PurposeDoxycycline-inducible mitochondria-localized ScaI expressing plasmidDepositorInsertScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-T2A-EGFP-Puro
Plasmid#234948PurposeDoxycycline-inducible mitochondria-localized ApaLI expressing plasmidDepositorInsertApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
UseLentiviralTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
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PE-pegRNA(BG)-nsgRNA(BG)
Plasmid#232727PurposeA dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).DepositorInsertpegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgNTC49-puro
Plasmid#231989PurposeKnockout non-targeting controlDepositorInsertsgRNA with Cas9 and hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgNTC13-hygro
Plasmid#231988PurposeKnockout non-targeting controlDepositorInsertsgRNA with Cas9 and hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-Il1a-ZsGreen
Plasmid#231987PurposeExpress IL-1alpha-ZsGreen fusion proteinDepositorInsertIL-1alpha-ZsGreen fusion construct
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3UTR
Plasmid#202550PurposeReady for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation mediaDepositorTypeEmpty backboneUseTagsATG initiation codon followed by an IGKV3 signal …ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5-toxic3
Plasmid#188625PurposepSC5GGv2 with HindII Gene InsertDepositorInsertHindII
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationACT1 intron insertPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA2ScIfm
Plasmid#193786PurposeExpresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTATAA synthetic promoter carrying binding sites…Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-CfANLN
Plasmid#183837PurposeRepair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.DepositorInsertCanis familiaris ANLN homology arms with mNeonGreen-linker
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only