We narrowed to 5,623 results for: pcas

1. pSR1038

   Plasmid

   #208737

   Purpose

   Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PCap
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCap

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX-evoCas9

   Plasmid

   #107550

   Purpose

   Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

   Depositor

   Anna Cereseto

   Article

   Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

   Insert

   Humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   M495V/Y515N/K526E/R661Q

   Promoter

   CBh

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGT6471

   Plasmid

   #219428

   Purpose

   Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   AGGT_NLS-SpCas9i-NLS(no stop)_TTCG
   

   Use

   Moclo compatible level 0 module

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pdg459-eSp(1.1) V3

   Plasmid

   #226965

   Purpose

   CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX459v3

   Plasmid

   #178799

   Purpose

   SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. gCBEv2

   Plasmid

   #220617

   Purpose

   vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   hUNG2-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

   Promoter

   hU6, EF1a

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. gTBEv3

   Plasmid

   #220619

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   hUNG2-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. gTBEv5

    Plasmid

    #220621

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. C-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58694

    Purpose

    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Csplit Intein and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. N-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58693

    Purpose

    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    3xFlag, GyrA Nsplit Intein, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. gTBEv4

    Plasmid

    #220620

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGG369

    Plasmid

    #165606

    Purpose

    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

    Expression

    Bacterial

    Mutation

    Recoding of BsaI site in the AmpR cassette, two B…

    Promoter

    lacUV2 driving ω-dCas9 and UV5 driving sgRNA

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXS-IRES-Blast FLVCR1b-3X FLAG

    Plasmid

    #218536

    Purpose

    human FLVCR1b with C-terminal 3X_FLAG cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    Retroviral

    Tags

    FLAG (3X)

    Expression

    Mammalian

    Mutation

    Alternative isoform

    Promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

    Plasmid

    #218534

    Purpose

    human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    Retroviral

    Tags

    FLAG (3X)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1+KDM5B-AS1

    Plasmid

    #194203

    Purpose

    Expresses lncRNA KDM5B-AS1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Insert

    KDM5B-AS1 (PCAT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pxCas9CR4

    Plasmid

    #111656

    Purpose

    PCas9CR4 with the xCas9 mutations allowing NG PAM sites

    Depositor

    Christopher Reisch

    Insert

    TetR and xCas9
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    xCas9 mutations

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB602-poUnaG

    Plasmid

    #220103

    Purpose

    For Agrobacterium-mediated transformation. Peroxisome-localized mCherry-UnaG.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

    Insert

    mCherry-UnaG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGWB602-ptUnaG

    Plasmid

    #221660

    Purpose

    For Agrobacterium-mediated transformation. Plastid(stroma)-localized mCherry-UnaG.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

    Insert

    mCherry-UnaG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGWB602-mtUnaG

    Plasmid

    #221664

    Purpose

    For Agrobacterium-mediated transformation. Mitochondria-localized mCherry-UnaG

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

    Insert

    mCherry-UnaG flanked with an N-terminal presequence of F1-ATPase γ-subunit
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGWB602-erUnaG

    Plasmid

    #221663

    Purpose

    For Agrobacterium-mediated transformation. Endoplasmic reticulum-localized mCherry-UnaG.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

    Insert

    mCherry-UnaG flanked with an N-terminal signal peptide or field pumpkin 25 albumin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB602-oeUnaG

    Plasmid

    #221662

    Purpose

    For Agrobacterium-mediated transformation. Plastid(outer envelope membrane)-localized mCherry-UnaG.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

    Insert

    mCherry-UnaG flanked with an N-terminal sequence of Arabidopsis OUTRE ENVELOPE PROTEIN7
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGWB602-tkUnaG

    Plasmid

    #221661

    Purpose

    For Agrobacterium-mediated transformation. Plastid(thylakoid membrane)-localized mCherry-UnaG.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

    Insert

    mCherry-UnaG flanked with the full-length coding sequence for Arabidopsis BESTROPHIN-LIKE PROTEIN2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIF1079

    Plasmid

    #237445

    Purpose

    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

    Depositor

    IIya Finkelstein

    Article

    Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

    Inserts

    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, H1, and U6

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSJX022

    Plasmid

    #232325

    Purpose

    Genome editing in Caulobacter crescentus

    Depositor

    Wei Zhao

    Article

    Sun et al bioRxiv 2024.12.02.626314

    Inserts

    sgRNA
    SpCas9M
    

    Use

    CRISPR

    Tags

    H-arms and cat

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119 and Pvan

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSJX024

    Plasmid

    #232327

    Purpose

    Genome editing in Caulobacter crescentus

    Depositor

    Wei Zhao

    Article

    Sun et al bioRxiv 2024.12.02.626314

    Inserts

    sgRNA
    SpCas9M
    

    Use

    CRISPR

    Tags

    H-arms and cat

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119 and Pxyl

    Available since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSJX016

    Plasmid

    #232324

    Purpose

    Genome editing in Caulobacter crescentus

    Depositor

    Wei Zhao

    Article

    Sun et al bioRxiv 2024.12.02.626314

    Inserts

    sgRNA
    SpCas9M
    

    Use

    CRISPR

    Tags

    H-arms and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119 and Pvan

    Available since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTW199b2

    Plasmid

    #136928

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge