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We narrowed to 22,796 results for: Sis

Showing: 3921 - 3960 of 22796 results

  1. pNOC_dfnCas12a_crRNA Td2

    Plasmid
    #176255
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176255-plasmid-map-sequence-id-347038

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  2. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4

    Plasmid
    #175815
    Purpose
    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tags
    Depositor
    Mark Banfield, Lennart Wirthmueller
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Promoter
    none
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175815-plasmid-map-sequence-id-346165

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  3. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2

    Plasmid
    #175816
    Purpose
    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tags
    Depositor
    Mark Banfield, Lennart Wirthmueller
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Mutation
    natural polymorphism P65S, annotated in Genbank f…
    Promoter
    none
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175816-plasmid-map-sequence-id-346164

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  4. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 9

    Plasmid
    #175817
    Purpose
    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 9 (AT5G03070.1) without stop codon for C-terminal epitope tags
    Depositor
    Mark Banfield, Lennart Wirthmueller
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 9 (IMPA-9 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Promoter
    none
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175817-plasmid-map-sequence-id-346163

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  5. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid
    #153441
    Purpose
    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode B knock-out mutant
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 140 and methionine 144 were mutated to a…
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153441-plasmid-map-sequence-id-306441

    Enlarge

  7. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  8. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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  10. pCAGGS-PHFF-sh-hBVRA

    Plasmid
    #100285
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100285-plasmid-map-sequence-id-194439

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  13. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Article
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    75143-plasmid-map-sequence-id-147264

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  14. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

    Enlarge

  15. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

    Enlarge

  16. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  18. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid
    #137885
    Purpose
    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Inserts
    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137885-plasmid-map-sequence-id-275856

    Enlarge

  20. pCAGGS-PHFF-sh-mBVRA

    Plasmid
    #100286
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available Since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100286-plasmid-map-sequence-id-194443

    Enlarge

  23. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

    Plasmid
    #175814
    Purpose
    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags
    Depositor
    Mark Banfield, Lennart Wirthmueller
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Promoter
    none
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175814-plasmid-map-sequence-id-346174

    Enlarge

  29. pMVP-SEC24C-G220C

    Plasmid
    #231560
    Purpose
    pMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (G220C) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    G220C, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231560-plasmid-map-sequence-id-460046

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  30. pMVP-SEC24C-S107F

    Plasmid
    #231561
    Purpose
    pMVP expression vector for human SEC24C (S107F mutation, closesd, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (S107F) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    S107F, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231561-plasmid-map-sequence-id-459737

    Enlarge

  32. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

    Enlarge

  35. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid
    #153440
    Purpose
    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode A knock-out mutant
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 191 and leucine 195 on XDocB were mutate…
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153440-plasmid-map-sequence-id-306440

    Enlarge

Showing: 3921 - 3960 of 22796 results