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Showing: 4901 - 4950 of 9181 results

  2. pTCF10 HLA-B*08:01

    Plasmid
    #180447
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*08:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180447-plasmid-map-sequence-id-355840

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  3. pE-SUMO CRYAA

    Plasmid
    #80756
    Purpose
    Expression of human alpha A-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYAA (CRYAA Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80756-plasmid-map-sequence-id-153571

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  4. pET11b-MIF Y99F

    Plasmid
    #149439
    Purpose
    Expression of MIF Y99F in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99F
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149439-plasmid-map-sequence-id-311578

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  5. rnALDH

    Plasmid
    #67920
    Purpose
    Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]

    Plasmid
    #100962
    Purpose
    Contains genes encoding GPP synthase, cineole synthase and IspA(S81F)
    Depositor
    Taek Soon Lee
    Insert
    GPPS-CSstr-ispA*
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100962-plasmid-map-sequence-id-193504

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  8. pE-SUMO CRYBA2

    Plasmid
    #80746
    Purpose
    Expression of human beta A2-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBA2 (CRYBA2 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80746-plasmid-map-sequence-id-153530

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  9. pET11b-MIF Y99A

    Plasmid
    #149440
    Purpose
    Expression of MIF Y99A in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99A
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149440-plasmid-map-sequence-id-311579

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  10. pAIP4_6IYE

    Plasmid
    #234237
    Purpose
    Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234237-plasmid-map-sequence-id-464201

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  11. pAIP4_1T3Y

    Plasmid
    #234228
    Purpose
    Heterologous protein expression of coactosin-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1T3Y (COTL1 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234228-plasmid-map-sequence-id-464193

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  12. pAIP4_2WWE

    Plasmid
    #234210
    Purpose
    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2WWE (PIK3C2G Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234210-plasmid-map-sequence-id-464175

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  13. pAIP4_3TOW

    Plasmid
    #234201
    Purpose
    Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3TOW (MVB12B Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234201-plasmid-map-sequence-id-464166

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  14. pAIP4_1KQX

    Plasmid
    #234181
    Purpose
    Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KQX (rbp2a Zebrafish)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234181-plasmid-map-sequence-id-464146

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  15. pAIP4_4NFB

    Plasmid
    #234161
    Purpose
    Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4NFB (PILRA Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234161-plasmid-map-sequence-id-464127

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  16. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

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  17. pET11b-MIF H62F

    Plasmid
    #149445
    Purpose
    Expression of MIF H62F in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H62F
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149445-plasmid-map-sequence-id-311584

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  18. pET11b-MIF H62A

    Plasmid
    #149443
    Purpose
    Expression of MIF H62A in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H62A
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149443-plasmid-map-sequence-id-311582

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  19. pET11b-MIF H62Y

    Plasmid
    #149442
    Purpose
    Expression of MIF H62Y in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H62Y
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149442-plasmid-map-sequence-id-311581

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  20. pET11b-MIF Y99G

    Plasmid
    #149441
    Purpose
    Expression of MIF Y99G in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99G
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149441-plasmid-map-sequence-id-311580

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  22. pET28a_LYN

    Plasmid
    #224238
    Purpose
    Expression of human LYN Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Use
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promotor
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224238-plasmid-map-sequence-id-458419

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  23. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Use
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

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  24. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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  25. R619-X67-527: His6-SARS-CoV-2 N

    Plasmid
    #170204
    Purpose
    E. coli expression of SARS-CoV-2 nucleocapsid protein
    Depositor
    Dominic Esposito
    Insert
    SARS-CoV-2 N (N SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170204-plasmid-map-sequence-id-334259

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  26. PAS_RvSAHS6

    Plasmid
    #215647
    Purpose
    Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS6
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215647-plasmid-map-sequence-id-426094

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  27. PAS_RvSAHS1

    Plasmid
    #215645
    Purpose
    Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS1
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215645-plasmid-map-sequence-id-426092

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  28. pTCF176 HLA-A*02:01 (CD8 mut)

    Plasmid
    #180459
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01 (CD8 mut)
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, T228A, replacement of transmembrane domain…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180459-plasmid-map-sequence-id-355125

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  29. pGEX-4T_CIP2A_1-560

    Plasmid
    #217921
    Purpose
    E. coli expression of GST-tagged CIP2A_1-560 protein
    Depositor
    Jukka Westermarck
    Insert
    CIP2A (CIP2A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    217921-plasmid-map-sequence-id-431066

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  30. pTCF79 H-2D(b) (CD8 mut)

    Plasmid
    #180453
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2D(b) (CD8 mut)
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, replacement of transmembrane domain with e…
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    180453-plasmid-map-sequence-id-409097

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  31. pE-SUMO CRYBA3

    Plasmid
    #80747
    Purpose
    Expression of human beta A3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBA3 (CRYBA1 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80747-plasmid-map-sequence-id-153503

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  32. pE-SUMO CRYGS

    Plasmid
    #80753
    Purpose
    Expression of human gamma S-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80753-plasmid-map-sequence-id-153560

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  34. pE-SUMO CRYBA1

    Plasmid
    #80745
    Purpose
    Expression of human beta A1-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBA1 (CRYBA1 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80745-plasmid-map-sequence-id-153525

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  35. pAIP4_3CX2

    Plasmid
    #234221
    Purpose
    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3CX2 (MYBPC3 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234221-plasmid-map-sequence-id-464186

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  36. pAIP4_4ACJ

    Plasmid
    #234212
    Purpose
    Heterologous protein expression of Wufb25h12 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ACJ (oxr1a Zebrafish)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234212-plasmid-map-sequence-id-464177

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  37. pAIP4_1EYH

    Plasmid
    #234159
    Purpose
    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EYH (Epn1 Rat)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234159-plasmid-map-sequence-id-464125

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  38. pET28a_HCK

    Plasmid
    #224239
    Purpose
    Expression of human HCK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    hck (HCK Human)
    Use
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promotor
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224239-plasmid-map-sequence-id-459258

    Enlarge

  39. PAS_HeSAHS4

    Plasmid
    #215648
    Purpose
    Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215648-plasmid-map-sequence-id-426093

    Enlarge

  40. PAS_RvSAHS4

    Plasmid
    #215646
    Purpose
    Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215646-plasmid-map-sequence-id-426091

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  41. pJA12 Mamu-A1*001:01

    Plasmid
    #180442
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*001:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    180442-plasmid-map-sequence-id-355627

    Enlarge

  42. pJA13 Mamu-A1*002:01

    Plasmid
    #180443
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-A1*002:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180443-plasmid-map-sequence-id-355622

    Enlarge

  43. pTCF122 Mamu-B*008:01

    Plasmid
    #180455
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-B*008:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180455-plasmid-map-sequence-id-355081

    Enlarge

  44. pTCF194 Mamu-E*02:04

    Plasmid
    #180461
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-E*02:04
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180461-plasmid-map-sequence-id-355080

    Enlarge

  45. pNK3071

    Plasmid
    #219755
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    219755-plasmid-map-sequence-id-450717

    Enlarge

  46. pNK497

    Plasmid
    #219754
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219754-plasmid-map-sequence-id-458646

    Enlarge

  47. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-215852

    Enlarge

  48. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

    Enlarge

Showing: 4901 - 4950 of 9181 results