We narrowed to 9,586 results for: Coli
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Plasmid#129587PurposeExpression of human INPP5A in bacteriaDepositorInsertType I inositol 5-phosphatase (INPP5A Human)
TagsMaltose binding proteinExpressionBacterialAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-Mit1AD
Plasmid#126721PurposeLacI-Mit1AD on pPICZ B expression vectorDepositorInserthybrid lacI-Mit1 activation domain coding sequence
ExpressionYeastPromoterICL1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-VP16
Plasmid#126723PurposeLacI-VP16 on pPICZ B expression vectorDepositorInserthybrid lacI-VP16 activation domain coding sequence
ExpressionYeastPromoterICL1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-Gal4AD
Plasmid#126724PurposeLacI-Gal4AD on pPICZ B expression vectorDepositorInserthybrid lacI-Gal4 activation domain coding sequence
ExpressionYeastPromoterICL1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-LM_lacO-cP-AOX1-sAR
Plasmid#126745Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
Tags6xHisExpressionYeastPromoterGAPAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-ICL1-LM_lacO-cP-AOX1-sAR
Plasmid#126744Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
Tags6xHisExpressionYeastPromoterICL1Available SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)
Plasmid#122450PurposeExpression of inositol 1,4,5-trisphosphate 3-kinase ADepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
Tagsbeta-galactosidaseExpressionBacterialMutationcoding sequence from V66 till stop codonAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), E166Q (EcN) MetNI LmH
Plasmid#118269PurposeN295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structureDepositorTags6x His TagExpressionBacterialMutationChanged Glutamic Acid (Glu) 166 to Glutamine (Gln…PromoterT7 PromoterAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500
Plasmid#92315PurposeExpression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL) mutant K16TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500
Plasmid#92227PurposeExpression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG D193TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidues Y35 D193 were mutated into TAG (Amber) s…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500
Plasmid#92230PurposeExpression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue V86 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T]
Plasmid#60753PurposeContains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.DepositorInsertsGalS-T
GalS-T
UseSynthetic BiologyExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…Available SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSm-Nuclease
Plasmid#132431PurposeExpresses Serratia marcescens nuclease in E. coli using T7 promoterDepositorInsertSerratia marcescens nuclease (codon optimized)
TagsNoneExpressionBacterialPromoterT7Available SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHyperTEV60
Plasmid#228496Purpose10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)DepositorInsertTEV Protease
Tags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyAvailable SinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneExpressionBacterialPromotertac promoter from pKK233-2Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL
Plasmid#62304PurposeIt is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.DepositorTypeEmpty backboneUseBaculovirus expressionTags6xHis and TEV cleavage sitePromoterpolyhedrinAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
YopH-pNIC-Bsa4
Plasmid#92063PurposepNIC expression vector encoding gene for YopHDepositorInsertYopH
Tags6x His TagExpressionBacterialMutationCodon optimized E. coliAvailable SinceJan. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
p28BIOH-LIC
Plasmid#62352PurposeE. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tagDepositorTypeEmpty backboneTags6xHis and AviTagExpressionBacterialPromoterT7-lacOAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMalAnPL
Plasmid#67281PurposeExpresses MBP-Anacystis nidulans photolyase in E. coliDepositorInsertphotolyase Anacystis nidulans
Tagsmaltose binding proteinExpressionBacterialMutationnone knownAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-NDFIP2
Plasmid#179330PurposeE. coli optimized expression of GST-fusion of human NDFIP2DepositorAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sc Rad27-pET24
Plasmid#239204PurposeOverexpress Sc Rad27 (FEN1) in E. coliDepositorInsertRad27 (RAD27 Budding Yeast)
ExpressionBacterialAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET/His-Rab1a(Q70L)
Plasmid#232919PurposeE. coli ExpressionDepositorAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2RH3
Plasmid#234219PurposeHeterologous protein expression of protein virC2 in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5Y33
Plasmid#234204PurposeHeterologous protein expression of alginate lyase in Escherichia coliDepositorInsert5Y33
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4REH
Plasmid#234195PurposeHeterologous protein expression of major latex-like protein in Escherichia coliDepositorInsert4REH
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4ZHW
Plasmid#234158PurposeHeterologous protein expression of YfiB in Escherichia coliDepositorInsert4ZHW (yfiB Pseudomonas aeruginosa PAO1)
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1XSZ
Plasmid#234151PurposeHeterologous protein expression of RalF in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2D5J
Plasmid#234143PurposeHeterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coliDepositorInsert2D5J
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
MZB186
Plasmid#228489PurposeEmpty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)-AvitagExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
C84m
Plasmid#120995PurposeMoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma 28
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHN764
Plasmid#178819PurposeExpression of EndoH in E. coliDepositorInsertsynthetic gene encoding endo-β-N-acetylglucosaminidase H
TagsHIs8 and MBPExpressionBacterialPromotertacAvailable SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialPromoterT7Available SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINN-GFP
Plasmid#53541PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
TagsN-His-GFPExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_SYK
Plasmid#224240PurposeExpression of human SYK Kinase in E. coliDepositorAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-pOsmB::mCherry
Plasmid#53313PurposemCherry reporter plasmid for OsmB promoterDepositorAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-pSoxS::mCherry
Plasmid#53311PurposemCherry reporter plasmid for SoxS promoterDepositorAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-rad24-pRSFDuet
Plasmid#240733PurposeOver-express FLAG-rad24 in E. coliDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN3
Plasmid#233520PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN4
Plasmid#233521PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1-10Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET/His-GST-Rab1a(Q70L)
Plasmid#232911PurposeE. coli ExpressionDepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3W4Q
Plasmid#234236PurposeHeterologous protein expression of beta-lactamase in Escherichia coliDepositorInsert3W4Q
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3ILS
Plasmid#234215PurposeHeterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coliDepositorInsert3ILS
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5NJO
Plasmid#234202PurposeHeterologous protein expression of putative cupin_2 domain-containing isomerase in Escherichia coliDepositorInsert5NJO
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits