Addgene

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Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-mCherry_puromycin

Plasmid

#65261

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-tagBFP

Plasmid

#65257

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNK875

Plasmid

#219735

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtTCTP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

AtTCTP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK928

Plasmid

#219736

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter del5_MtHP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter of MtHP gene from Medicago truncatula, del5_MtHP

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

del5_MtHP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1198

Plasmid

#219740

Purpose

MoClo-compatible Level 0 - 3U + terminator vector carrying terminator HSP18.2

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

The heat shock protein 18.2 (HSP) terminator

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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Nc-NylC_pET28a

Plasmid

#215412

Purpose

Expression construct for Nc-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Nc-NylC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2537

Plasmid

#195658

Purpose

Complementation of MlaC(R153A)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(R153A)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2538

Plasmid

#195659

Purpose

Complementation of MlaC(R186A)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(R186A)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2539

Plasmid

#195660

Purpose

Complementation of MlaC(T116D)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(T116D)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2540

Plasmid

#195661

Purpose

Complementation of MlaC(T176R)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(T176R)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2541

Plasmid

#195662

Purpose

Complementation of MlaC(V68R)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(V68R)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2542

Plasmid

#195663

Purpose

Complementation of MlaC(V135R)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(V135R)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2543

Plasmid

#195664

Purpose

Complementation of MlaC(V146R)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(V146R)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2544

Plasmid

#195665

Purpose

Complementation of MlaC(V171R)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(V171R)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2545

Plasmid

#195666

Purpose

Complementation of MlaC(Y72A)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(Y72A)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2546

Plasmid

#195667

Purpose

Complementation of MlaC(Y82K)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(Y82K)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2547

Plasmid

#195668

Purpose

Complementation of MlaC(Y105A)

Depositor

Damian Ekiert, Gira Bhabha

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

MlaC(Y105A)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge