We narrowed to 165,567 results for: addgene

1. LentiGuide-TagBFP-2A-BSD

   Plasmid

   #167929

   Purpose

   Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTB3-BC2 nanobody

   Plasmid

   #210208

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   BC2 Nanobody
   

   Expression

   Bacterial

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL1P3 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198047

   Purpose

   MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available Since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIRE

   Plasmid

   #26745

   Depositor

   Rolf Zeller

   Article

   Osterwalder et al Nat Methods. 2010 Oct 17

   Insert

   iCre expression cassette
   

   Use

   Cre/Lox; Flp/frt

   Expression

   Mammalian

   Available Since

   Jan. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-LSD1-Hygro

   Plasmid

   #104406

   Purpose

   transient expression of dCas9-LSD1 fusion protein

   Depositor

   Stephan Beck

   Insert

   LSD1 (KDM1A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   IRATp970A0364D (Source Bioscience) has a P405H ch…

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p8266 LentiCRISPR v2 hygro sgNT-1

   Plasmid

   #193977

   Purpose

   Expression of spCas9 and non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniCMV-iRFP670-24xMS2

   Plasmid

   #238915

   Purpose

   Contains miniCMV promoter expressing iRFP670 mRNA taged 24xMS2 motif

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

   Insert

   iRFP670-24xMS2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. LacZ_pHIVEGFP

   Plasmid

   #191251

   Purpose

   Expresses LacZ in mammalian cells (used as a control)

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

   Insert

   LacZ
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-puro-shFAM134B

   Plasmid

   #109013

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   shFAM134B (RETREG1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. E[beta]C

    Plasmid

    #24312

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. HLA_A0101

    Plasmid

    #165009

    Purpose

    Expression of HLA-A*01:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-A*01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACUH

    Plasmid

    #58374

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Yuh-Nung Jan, Chun Han

    Article

    Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. 15x-QUAS-dTomato-T2A-GCaMP6s

    Plasmid

    #130666

    Purpose

    QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    15xQUAS-dTomato-T2A-GCaMP6s
    

    Use

    Ae. aegypti expression

    Expression

    Insect

    Promoter

    15xQUAS-TATA

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBI-MCS-EYFP

    Plasmid

    #58855

    Purpose

    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    enhanced yellow fluorescent protein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTlpA_mCherry

    Plasmid

    #229146

    Purpose

    Promoter strength evaluation of PtlpA

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry
    

    Expression

    Bacterial

    Mutation

    No

    Promoter

    PtlpA

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCrhaB2plus

    Plasmid

    #164226

    Purpose

    Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

    Depositor

    Silvia Cardona

    Article

    Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Rhamnose-inducible

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPE4max-Blasticidin

    Plasmid

    #194905

    Purpose

    Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

    Depositor

    George Church

    Article

    Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

    Insert

    BSD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GalToxBFP

    Plasmid

    #68073

    Purpose

    mammalian expression of GC localized oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    GalT signal anchor transmembrane domain
    

    Tags

    moxBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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