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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,248 results for: cat.3

Showing: 1961 - 1980 of 3248 results

  1. pKK-TEV-MBP

    Plasmid
    #105787
    Purpose
    Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-MBP
    Expression
    Mammalian
    Available Since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105787-plasmid-map-sequence-id-211379

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  2. Cntn3-AP-His

    Plasmid
    #71941
    Purpose
    Expresses the extracellular region of the Contactin 3 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Cntn3 (Cntn3 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71941-plasmid-map-sequence-id-135566

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  3. pCMK188

    Plasmid
    #226659
    Purpose
    pCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 site
    Depositor
    David Nielsen
    Type
    Empty backbone
    Use
    Unspecified
    Available Since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    226659-plasmid-map-sequence-id-455439

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  4. pCMK191

    Plasmid
    #226658
    Purpose
    Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site
    Depositor
    David Nielsen
    Type
    Empty backbone
    Use
    Unspecified
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    226658-plasmid-map-sequence-id-461184

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  5. pCMK74

    Plasmid
    #226657
    Purpose
    Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, KanR, contains HIP1 site
    Depositor
    David Nielsen
    Type
    Empty backbone
    Use
    Unspecified
    Available Since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    226657-plasmid-map-sequence-id-455440

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  7. pLSU1/t35s[TMV]:mNeonGreen-CI{PDK-198}:NosT

    Plasmid
    #212176
    Purpose
    This binary vector expresses mNeonGreen containing the catalase (PDK) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PDK-198}
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PDK int…
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212176-plasmid-map-sequence-id-422742

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  12. pAAV 3xgRNA KO;Rbfox3

    Plasmid
    #240311
    Purpose
    KO:NeuN
    Depositor
    Yuki Ogawa
    Insert
    KO gRNAs for NeuN
    Use
    AAV
    Mutation
    NA
    Available Since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240311-plasmid-map-sequence-id-478214

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  13. pINER2R

    Plasmid
    #185882
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185882-plasmid-map-sequence-id-370829

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  14. pIT7A6

    Plasmid
    #185896
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185896-plasmid-map-sequence-id-370384

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  18. pBP-T7_RBS-His6-Thrombin

    Plasmid
    #72979
    Purpose
    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS+His6+thrombin
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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Showing: 1961 - 1980 of 3248 results