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Showing: 121 - 140 of 6920 results

  1. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Availability
    Academic Institutions and Nonprofits only

  2. pDBD-M

    Plasmid
    #50572
    Purpose
    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal1
    Availability
    Academic Institutions and Nonprofits only

  9. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    yeast ADH1 promoter
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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  10. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Yeast ADH1 promoter
    Availability
    Academic Institutions and Nonprofits only
    51056-plasmid-map-sequence-id-77326

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  13. pRS316-RGR-GFP-mm

    Plasmid
    #51059
    Purpose
    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Yeast ADH1 promoter
    Availability
    Academic Institutions and Nonprofits only
    51059-plasmid-map-sequence-id-77330

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  14. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    pTPGI (galactose+aTc inducible)
    Availability
    Academic Institutions and Nonprofits only
    49013-plasmid-map-sequence-id-231494

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Showing: 121 - 140 of 6920 results