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Showing: 1 - 20 of 9833 results
  1. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EFS
    Availability
    Academic Institutions and Nonprofits only
  2. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EFS
    Availability
    Academic Institutions and Nonprofits only
  3. lentiCRISPR - EGFP sgRNA 3

    Plasmid
    #51762
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EFS
    Availability
    Academic Institutions and Nonprofits only
  4. lentiCRISPR - EGFP sgRNA 4

    Plasmid
    #51763
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EFS
    Availability
    Academic Institutions and Nonprofits only
  5. lentiCRISPR - EGFP sgRNA 5

    Plasmid
    #51764
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mutation
    Promoter
    U6 and EFS
    Availability
    Academic Institutions and Nonprofits only
  6. lentiCRISPR - EGFP sgRNA 6

    Plasmid
    #51765
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EFS
    Availability
    Academic Institutions and Nonprofits only
  7. pUC119-gRNA

    Plasmid
    #52255
    Purpose
    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
    Depositor
    Insert
    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis U6 polymerase III promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    constitutive 35SPPDK promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Insert
    Kanamycin cassette
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  10. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    Toxoplasma U6 and TgTUB1
    Availability
    Academic Institutions and Nonprofits only
  11. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Has Service
    Cloning Grade DNA
    Inserts
    Cas9
    Puromycin resistance
    Use
    Lentiviral and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Availability
    Academic Institutions and Nonprofits only
  12. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    Lentiviral and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Availability
    Academic Institutions and Nonprofits only
  13. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    Lentiviral and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 9833 results