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Plasmid#68702PurposeNeisseria meningitidis Cas9DepositorInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX394 Neisseria cinerea Cas9
Plasmid#68331PurposeNeisseria cinerea Cas9DepositorInsertNcCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVD300recA6
Plasmid#37062DepositorInsertRecA (recA Neisseria gonorrhoeae (Gc))
UseInducible gc expression vectorTagsExpressionMutationPromoterAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Spacer Switch Plasmid
Plasmid#215346PurposeFacilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.DepositorInsertOpaD spacer; Chloramphenicol Resistance Cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ WT
Plasmid#129647PurposeExpresses the mature metQ gene from Neisseria meningitides, without the signal sequenceDepositorInsertmetQ Gene
UseTags10x Histidine Tag, Enterokinase, and pelB signal …ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ N238A
Plasmid#129648PurposeExpresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequenceDepositorInsertmetQ Gene
UseTags10x Histidine Tag, Enterokinase, and pelB signal …ExpressionBacterialMutationChanged Asparagine 238 to AlaninePromoterT7 PromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-Wt-NmeCas9
Plasmid#71474Purposebacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-WT-NmeCas9
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYZ1389
Plasmid#215348PurposeNla CRISPRi Delivery VectorDepositorInsertNla CRISPRi Genes (Cas5, Cas8c, Cas7)
UseCRISPR; Nla i delivery vectorTagsExpressionMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-dNmeCas9
Plasmid#71475Purposebacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-Inactive-NmeCas9
UseTags6HisExpressionBacterialMutationD16A H588APromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS654 All-in-One AAV-sgRNA-hNmeCas9
Plasmid#112139PurposeDelivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.DepositorInsertssgRNA scaffold
Human-codon-optimized NmeCas9
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMammalianMutationPromoterU1a and U6Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-H588A-NmeCas9
Plasmid#71477Purposebacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-H588A-NmeCas9
UseTags6HisExpressionBacterialMutationH588APromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-D16A-NmeCas9
Plasmid#71476Purposebacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-D16A-NmeCas9
UseTags6HisExpressionBacterialMutationD16APromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag
Plasmid#109030PurposeWild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A
Plasmid#109031PurposeD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-T7-NlaCas3-NLS-6xHis
Plasmid#180214PurposeExpressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.ColiDepositorInsertNlaCas3c
UseTags6xHis and NLSExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS433-pCSDest2-AcrIIC1Nme
Plasmid#85679PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC1Nme
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS436-pCSDest2-AcrIIC2Nme
Plasmid#85712PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC2Nme
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS443-pCSDest2-AcrIIC3Nme
Plasmid#85713PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC3Nme
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only