Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 121 - 140 of 840 results

  1. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

    Enlarge

  2. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

    Enlarge

  3. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

    Enlarge

  4. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

    Enlarge

  5. pX458-tRNA-SpCas9

    Plasmid
    #195183
    Purpose
    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.
    Depositor
    Stephanie Roessler
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    tRNA-Gln
    Availability
    Academic Institutions and Nonprofits only
    195183-plasmid-map-sequence-id-423143

    Enlarge

  6. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Expression
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

    Enlarge

  19. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

    Enlarge

  20. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

    Enlarge

Showing: 121 - 140 of 840 results