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pBS-AMV-γ1L9'S-nAchR

Plasmid

#20686

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrng Mouse)

Use

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Promoter

Availability

Academic Institutions and Nonprofits only
pBS-AMV-δ1L9'S-nAchR

Plasmid

#20687

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors

Use

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Promoter

Availability

Academic Institutions and Nonprofits only
LLP338 pEF1a-DACH1-1-SunTag

Plasmid

#100942

Purpose

TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for DACH1, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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alpha7-nAChR(scramble control)

Plasmid

#86649

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNA7 (control)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

Plasmid

#137960

Purpose

Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR (non-binding yellow variant)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Y140A binding site mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

3xMnx1

Availability

Academic Institutions and Nonprofits only

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pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-bReaches-TS

Plasmid

#158701

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-bReaches-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N425/45R-2b]

Plasmid

#206672

Purpose

Mammalian Expression Plasmid of anti-VAChT vesicular acetylcholine transporter (Rat). Derived from hybridoma N425/45-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT vesicular acetylcholine transporter (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW Achilles-T2A-mCherry

Plasmid

#205499

Purpose

Expression of Achilles-T2A-mCherry in yeast

Depositor

Bas Teusink

Insert

Achilles-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N425/45R-1]

Plasmid

#211600

Purpose

Mammalian Expression Plasmid of anti-VAChT (Rat) subclass-switched IgG1 R-mAb. Derived from hybridoma N425/45.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc18a3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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hSyn-FAS-DO-ReaChR-mCitrine-WPRE

Plasmid

#64035

Purpose

Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs

Depositor

Karel Svoboda

Insert

hSyn-FAS-DO-ReaChR-mCitrine-WPRE

Use

AAV

Tags

ReaChR tagged with mCitrine

Expression

Mammalian

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-his-BACH1 K52R

Plasmid

#17643

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

The lysine residue at position 52 was changed to …

Promoter

Availability

Academic Institutions and Nonprofits only
pCDNA3-myc-his-BACH1 P47A

Plasmid

#17644

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

To generate the tumor-associated mutant, the prol…

Promoter

Availability

Academic Institutions and Nonprofits only
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

Plasmid

#71829

Purpose

Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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beta4-nAChR shRNA (Beta-4.3)

Plasmid

#86650

Purpose

expression of shRNA targeting CHRNB4

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (beta4-nAChR) (CHRNB4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Availability

Academic Institutions and Nonprofits only

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beta4-nAChR (DPM negative control)

Plasmid

#86651

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (control)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Availability

Academic Institutions and Nonprofits only

Enlarge
alpha4-nAChR shRNA (Alpha-4.3)

Plasmid

#86652

Purpose

expression of shRNA targeting CHRNA4

Depositor

Jaime Modiano

Insert

CHRNA4 (alpha4-nAChR) (CHRNA4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Availability

Academic Institutions and Nonprofits only

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alpha3-nAChR shRNA (Alpha-3.29)

Plasmid

#86655

Purpose

expression of shRNA targeting CHRNA3

Depositor

Jaime Modiano

Insert

CHRNA3 (alpha3-nAChR) (CHRNA3 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Availability

Academic Institutions and Nonprofits only

Enlarge