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Plasmid#13840DepositorInsertPPAT
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGLU_63
Plasmid#225766PurposeThis is the native promoter from the tetL tetracycline resistance cassette.DepositorInsertPtetL_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-EbpA_g1
Plasmid#153517PurposesgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertpnisA-sgRNA(EbpA_g1)-dCas9 scaffold
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRecT_2
Plasmid#167546PurposeEncodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.DepositorInsertE. faecium Com15 recT under an IPTG inducible promoter, with lacI
UseTagsExpressionBacterialMutationPromoterIPTGAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL0_166 [Taad(9)]
Plasmid#210686PurposeLevel 0 partDepositorInsertTaad(9)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_165 [aad(9)]
Plasmid#210685PurposeLevel 0 partDepositorInsertaad(9)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_164 [Paad(9)]
Plasmid#210684PurposeLevel 0 partDepositorInsertPaad(9)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115268PurposeExpresses EiCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertEiCsm6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Mev1
Plasmid#65815PurposeNADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli ExpressionDepositorInsertsmvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
mvaS: hydroxymethylglutaryl-CoA synthase
UseTagsExpressionBacterialMutationPromoterinsulated mipAp from E. coli and insulated waaHp…Available sinceSept. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGPA1
Plasmid#115476PurposeGeneration of mariner-based transposon mutant libraries in Enterococcus faeciumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC005.em
Plasmid#182738PurposeCRISPR-Cas12a genome editing of E. faeciumDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJC005.gent
Plasmid#182739PurposeCRISPR-Cas12a genome editing of E. faeciumDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-PMK
Plasmid#108949Purposeheterologous expression of PMK of E. faecalis in E. coliDepositorInsertPhosphomevalonate kinase (PMK)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-HMGS
Plasmid#108945Purposeheterologous expression of HMGS of E. faecalis in E. coliDepositorInsertHMG-CoA synthase (HMGS)
UseTagsHis6-TagExpressionBacterialMutationA110GPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCsRNAP
Plasmid#182746PurposeSwapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmidsDepositorInsertsmall RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEXndoE
Plasmid#47714PurposeExpresses GST-EndoE from E. faecalis in E. coliDepositorInsertndoE
UseTagsGlutathione S-transferaseExpressionBacterialMutationPromotertacAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL0_57 [PtetL]
Plasmid#198970PurposeLevel 0 partDepositorInsertPtetL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_58 [tetL]
Plasmid#198971PurposeLevel 0 partDepositorInserttetL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only