Showing: 41 - 60 of 62 results
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pRR-ERalpha-5Z
Plasmid#23061DepositorInsertERalpha (ESR1 Human)
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS_FLPER(T)
Plasmid#31131DepositorInsertFLP-ER ligand binding domain (ESR1 Budding Yeast, Human)
UseTagsExpressionMammalianMutationfull length FLP linked to ligand binding domain o…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS_FLPER(T2)
Plasmid#31303DepositorInsertFLP-ER ligand binding domain (ESR1 Budding Yeast, Human)
UseTagsExpressionMammalianMutationfull length FLP linked to ligand binding domain o…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-ER alpha
Plasmid#28230PurposeMammalian expression of Estrogen Receptor alpha fused to EGFPDepositorInsertEstrogen Receptor alpha (ESR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBMN ER50-YFP
Plasmid#37259DepositorInsertER50 (ESR1 Human)
UseRetroviralTagsYFP-HAExpressionMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…PromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
PBMN YFP-ER50
Plasmid#37261DepositorInsertER50 (ESR1 Human)
UseRetroviralTagsHA-YFPExpressionMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…PromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
pHES795
Plasmid#87943PurposeConstitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Human, Synthetic)
UseTagsExpressionYeastMutationPromoterADH1 (crippled)AvailabilityAcademic Institutions and Nonprofits only -
VP16-ER alpha
Plasmid#11351DepositorInsertER alpha (ESR1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
nERb446-gs10-NLS-VPR
Plasmid#188263PurposePlasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnERb446 (ESR1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Human, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterADH1 (crippled)AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode
Plasmid#170225PurposeBarcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertER (ESR1 Human)
UseLentiviralTagsV5ExpressionMutationY537SPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cERb447
Plasmid#188257PurposePlasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcERb447 (ESR1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
3HA-ERHBD-Hoxb8
Plasmid#212932PurposeExpresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell linesDepositorUseRetroviralTagsTriple HA tagExpressionMammalianMutationPromoterMSCV LTRAvailabilityAcademic Institutions and Nonprofits only -
pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN
Plasmid#99049PurposeTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domainDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-hERalpha
Plasmid#101141PurposeMammalian expression of full-length human estrogen receptor-alphaDepositorInsertestrogen receptor-alpha (ESR1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHEY1-donor
Plasmid#112297PurposeCRISPR donor plasmid to tag human transcription factor HEY1 with GFPDepositorInsertHEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
UseCRISPRTagsExpressionMutationhg38:8:79765311:G>APromoterAvailabilityAcademic Institutions and Nonprofits only -
pHEY1.1.0-gDNA
Plasmid#112393PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1DepositorInsertHEY1 (HEY1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
TFORF1507
Plasmid#144180PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHEY1 (HEY1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
TFORF3136
Plasmid#144612PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHEY1 (HEY1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 62 results