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Showing: 21 - 40 of 72 results

  1. HRH1-DuET

    Plasmid
    #213312
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HRH1 (HRH1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213312-plasmid-map-sequence-id-430850

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  2. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid
    #190112
    Purpose
    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Inserts
    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    Use
    AAV
    Tags
    bpNLS and bpNLS-P2A-eGFP
    Expression
    Mutation
    mutations from RT in PE2 and truncation of RNAse …
    Promoter
    EFS and U6, H1
    Availability
    Academic Institutions and Nonprofits only
    190112-plasmid-map-sequence-id-372717

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  5. PTPN3

    Plasmid
    #38966
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN3 (PTPN3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  12. pLVUT-tTR-KRAB

    Plasmid
    #11651
    Purpose
    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation
    Depositor
    Patrick Aebischer, Didier Trono
    Article
    Szulc et al Nat Methods. 2006 Feb . 3(2):109-16.
    Has Service
    Cloning Grade DNA
    Insert
    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    11651-plasmid-map-sequence-id-217481

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Showing: 21 - 40 of 72 results