We narrowed to 217 results for: multiplex

1. pU1/Sm/3' Box_(GLuc)_TOP1

   Plasmid

   #68437

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U1

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

   Plasmid

   #192683

   Purpose

   Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV/3' Box_(GLuc)_TOP2

   Plasmid

   #68435

   Purpose

   Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP2 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6_(GLuc)_INT[Spinach2]

   Plasmid

   #68430

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Spinach2" aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

   Plasmid

   #192676

   Purpose

   Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV/MASC_(GLuc)_INT

   Plasmid

   #68440

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6_(GLuc)_TOP1

   Plasmid

   #68423

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-sgSetd2#1/Cre

   Plasmid

   #89649

   Purpose

   Expresses a Setd2-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Setd2 (Setd2 Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

   Plasmid

   #192681

   Purpose

   Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEE389

    Plasmid

    #149287

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pEE385

    Plasmid

    #149289

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-sgSetd2#2/Cre

    Plasmid

    #89650

    Purpose

    Expresses a Setd2-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Setd2 (Setd2 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6_(GLuc)_INT[GFPApt]

    Plasmid

    #68428

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the GFP aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6_(GLuc)_INT[3xK-T]

    Plasmid

    #68429

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three kink-turns
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SL3-MS2-gRNA GG backbone (MOBE3-4)

    Plasmid

    #219949

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-SL3 (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3'-com-gRNA GG backbone (MOBE1-4)

    Plasmid

    #219947

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-com-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3'MS2-gRNA GG backbone (MOBE1-2)

    Plasmid

    #219948

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

    Plasmid

    #194725

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6_(GLuc)_INT[S1Apt]

    Plasmid

    #68425

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct with S1 streptavidin aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDA_550

    Plasmid

    #203398

    Purpose

    All-in-one lentiviral expression of gRNA and EnAsCas12a

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Insert

    Enhanced Acidaminococcus Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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