Showing: 41 - 60 of 686 results
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Plasmid#99988PurposeExpress yeast Esf1 with Y2H activation domain fusionDepositorInsertEsf1 (ESF1 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ssf1
Plasmid#63778Purposeyeast ORF Ssf1 in the Gateway entry vector pDONR221DepositorInsertSsf1
UseGateway donor vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1aMS2p65HSF1hyg-W
Plasmid#112920PurposeLentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoterDepositorInsertMS2p65HSF1-T2Ahyg
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman EF1a promoterAvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-FSF1
Plasmid#110641PurposeRetroviral expression of flag-tagged yeast FSF1 in mammalian cellsDepositorInsertFSF1 (FSF1 Budding Yeast)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-HTATSF1__BRS3
Plasmid#116822PurposeLentiviral expression of HTATSF1__BRS3 fusionDepositorInsertHTATSF1__BRS3
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-TNFRSF10B
Plasmid#117373PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR TNFRSF10B
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80R
Plasmid#118350PurposePlasmid expresses hHSF1 with K80 in DBD mutated to R.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK80RPromoterAvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80Q
Plasmid#118351PurposePlasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK80QPromoterAvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K298R
Plasmid#118362PurposeThis plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK298RPromoterAvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303A
Plasmid#118363PurposeThis plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303APromoterAvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S307A
Plasmid#118364PurposeThis plasmid expresses human HSF1 S307A mutant.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS307APromoterAvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303/7A
Plasmid#118365PurposeThis plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303A and S307APromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR1A dn-cHSF1
Plasmid#134724PurposeGateway entry vector for constitutively active dominant-negative HSF1DepositorInsertdn-cHSF1 (HSF1 Human)
UseEntry vector for gateway cloningTagsExpressionMutationDeletion of amino acids 186-202, 379−529PromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pENTR1A DHFR.cHSF1
Plasmid#134737PurposeGateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseEntry vector for gateway cloningTagsDHFRExpressionMutationDeletion of amino acids 186-202PromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pSF1C-A-RFP
Plasmid#172431PurposeStrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP2
Plasmid#178969PurposeExpresses miRFP2 in worm cellsDepositorInsertwmiRFP2
UseTagsnoExpressionWormMutationNOPromotertag-168 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSF11-wElectra1
Plasmid#184932PurposeCodon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmidDepositorInsertElectra1
UseTagsExpressionWormMutationPromotertag-168AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wElectra2
Plasmid#184933PurposeCodon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmidDepositorInsertElectra2
UseTagsExpressionWormMutationPromotertag-168AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmTagBFP2
Plasmid#184934PurposeCodon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmidDepositorInsertmTagBFP2
UseTagsExpressionWormMutationPromotertag-168AvailabilityAcademic Institutions and Nonprofits only -
pST_B4YSF1_9LAMI (pBS0347)
Plasmid#185186PurposeFor the mammalian expression of the Dorcoceras hygrometricum protein B4YSF1_9LAMI. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertB4YSF1_9LAMI
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 686 results