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pDONR223_TNFAIP3_WT
Plasmid#82238PurposeGateway Donor vector containing TNFAIP3 , part of the Target Accelerator Plasmid Collection.DepositorInsertTNFAIP3 (TNFAIP3 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-TNFalpha
Plasmid#20210DepositorInsertTNFa 3' UTR (TNF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-TNFalpha
Plasmid#28089DepositorInsertTNFalpha (Tnf Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-TNFa-shRNA3
Plasmid#72598PurposeExpress shRNA against mouse TNF-alphaDepositorInsertmouse TNF-alpha shRNA (Tnf Mouse)
UseRNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
mApple-TNFa-RUSH
Plasmid#166902PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mAppleDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-TNFa-RUSH
Plasmid#166901PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-HaloDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H
Plasmid#108374PurposeExpresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)
Plasmid#230024PurposeExpresses anti-TNFalpha PAGER(FL) in mammalian cellsDepositorInsertpAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)
Plasmid#230015PurposeExpresses anti-TNFalpha PAGER(Gq) in mammalian cellsDepositorInsertMT1-Ozoralizumab-TEVcs-ALFA-hM1D
UseAAVTagsExpressionMammalianMutationW442A on hM1DPromoterCMVAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)
Plasmid#230001PurposeExpresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH
Plasmid#148878PurposeMammalian Expression of TNFalpha-ARE-polyA-MalatDepositorInsertTNFalpha-ARE-polyA-Malat (TNF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP
Plasmid#223214PurposeLentiviral vector - mCherry reporter and sTNFaR for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertSoluble TNFαR (TNFRSF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-RpHLuorin2
Plasmid#171722PurposeSynchronize trafficking of TNFa-RpHLuorin2DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and ratiometri…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNF-Fc(DAPA)-AviTag-6xHis
Plasmid#156575PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNF (TNF Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNF-COMP5AP-AviTag-9xHis
Plasmid#157140PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNF (TNF Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR
Plasmid#82024PurposeLentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selectionDepositorInsertmKate2-2xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR
Plasmid#81255PurposeLentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
k3(long) luciferase
Plasmid#11110DepositorInsertk3(L) (TNF Human)
UseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TNF
Plasmid#67062PurposeStandard for cell-free expression benchmarking.DepositorInsertTNF (TNF Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only
