We narrowed to 46 results for: wolberger

1. FLAG-OTUB1 WT-pcDNA3.1

   Plasmid

   #118209

   Purpose

   Expresses FLAG-OTUB1 WT in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-UBE2E1 WT in pcDNA3.1

   Plasmid

   #118213

   Purpose

   Expresses HA-UBE2E1 in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6TEV-UbG76C

   Plasmid

   #75299

   Purpose

   Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   human Ubiquitin mutant G76 to C
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Glycine 76 to Cystine

   Promoter

   T7

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. UbcH5A

   Plasmid

   #61081

   Purpose

   express a human E2

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   UbcH5A (UBE2D1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. hUbc13

   Plasmid

   #51131

   Purpose

   human ubc13, E2 for synthesis k63 linked ubiquitin chain

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

   Insert

   ubc13 (UBE2N Human)
   

   Tags

   His6 and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. SENP2M497A

   Plasmid

   #61088

   Purpose

   cleave the SUMO off when use PETSUMO vector for express fusion protein

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   SENP2M497A catalytic domain (SENP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   M497A; Catalytic domain is residues 364-589

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pEW108

   Plasmid

   #232823

   Purpose

   Expresses yeast compass complex in insect cells

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

   Insert

   COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
   

   Tags

   His6-3xFLAG, Twinstrep

   Expression

   Insect

   Mutation

   Set1(762-1080)

   Promoter

   polyhedrin promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nampt-PET28a

   Plasmid

   #25630

   Depositor

   Cynthia Wolberger

   Article

   Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

   Insert

   Nampt (Nampt Mouse)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-OTUB1 C91S-pcDNA3.1

    Plasmid

    #118210

    Purpose

    Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 91 to serine

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. UbcH5B

    Plasmid

    #60443

    Purpose

    Express E2 UbcH5B in E. Coli

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    UbcH5B (UBE2D2 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. HA-UBE2E1 C131A in pcDNA3.1

    Plasmid

    #118214

    Purpose

    Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    UBE2E1 (UBE2E1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed cysteine 131 to alanine

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. H2BK120C

    Plasmid

    #75332

    Purpose

    Bacteria expression of H2BK120C

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    H2BK120C (h2bc11.S Frog)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    truncated; K120C

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEW3: pET-32a Dot1L(2-416)

    Plasmid

    #124098

    Purpose

    Dot1L(2-416) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-416) (DOT1L Human)
    

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UBC13-PGEX4T

    Plasmid

    #25464

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    Ubc13 (UBC13, MMS2 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-OTUB1 T134R-pcDNA3.1

    Plasmid

    #118211

    Purpose

    Expresses FLAG-OTUB1 T134R mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 134 to arginine

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MBP-His6-TEV-MMS2

    Plasmid

    #25465

    Depositor

    Cynthia Wolberger

    Article

    Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

    Insert

    MMS2 (MMS2, UBC13 Budding Yeast)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    July 21, 2010

    Availability

    Academic Institutions and Nonprofits only

18. hOtub1

    Plasmid

    #26959

    Depositor

    Cynthia Wolberger

    Article

    Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

    Insert

    Otub1 (OTUB1 Human)
    

    Tags

    His6 (TEV cleavable)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pTXB1-H2B(1-114)_wt

    Plasmid

    #118252

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEW4: pET-32a Dot1L(2-386)

    Plasmid

    #124099

    Purpose

    Dot1L(2-386) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-386) (DOT1L Human)
    

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge