388 results

1. Human sgRNA library Brunello in lentiCRISPRv2

   Pooled Library

   #73179

   Purpose

   Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Has Service

   Lentiviral Prep

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

2. Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)

   Pooled Library

   #1000000052

   Purpose

   CRISPR gRNA knockout pooled library for targeting the mouse genome

   Depositor

   Feng Zhang

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Inzolia library in pRDA_550

   Pooled Library

   #209552

   Purpose

   The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

4. Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone

   Pooled Library

   #1000000083

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human kinome

   Depositor

   John Doench, David Root

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

5. Human Epigenetic Knockout Library

   Pooled Library

   #162256

   Purpose

   Knockout library that targets human epigenetic genes.

   Depositor

   Kivanc Birsoy

   Article

   Williams et al Cell Metab. 2020 Apr 7;31(4):852-861.e6.

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

6. Jacquere (split-vector)

   Pooled Library

   #247026

   Purpose

   Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

   Depositor

   John Doench, David Root

   Article

   Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

7. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)

   Pooled Library

   #1000000111

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of human genes

   Depositor

   John Doench, David Root

   Available Since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

8. Lenti-human-TF-gRNA-puromycin

   Pooled Library

   #162275

   Purpose

   Knockout library targeting a set of human transcription factors for loss-of-function screening.

   Depositor

   Chunliang Li

   Article

   Zhang et al Elife. 2020 Oct 1;9. pii: 57858. doi: 10

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

9. FNLCR CRISPRa Cell Surface

   Pooled Library

   #207471

   Purpose

   CRISPR-based activation library with guide RNAs targeting all cell surface proteins.

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

10. Human genome-wide lentiviral CRISPR gRNA library version 1

    Pooled Library

    #67989

    Purpose

    Knockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Species

    Homo sapiens, Mus musculus, and Synthetic

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Mouse Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187081

    Purpose

    Library targeting 2,989 mouse metabolic genes for activation of gene expression.

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

12. Human sgRNA library Gattinara in pRDA_118 backbone

    Pooled Library

    #136986

    Purpose

    Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

13. DNA repair library with 1,573 sgRNAs

    Pooled Library

    #177663

    Purpose

    CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Species

    Homo sapiens

    Use

    CRISPR

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

14. MORF Library Kit with GFP and mCherry controls

    Pooled Library

    #1000000218

    Depositor

    Feng Zhang

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

15. Human sgRNA library Brunello in lentiGuide-Puro

    Pooled Library

    #73178

    Purpose

    Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

16. RLC12

    Pooled Library

    #163954

    Purpose

    Targets open reading frames and non-coding RNAs in M. tuberculosis.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Expression

    Bacterial

    Use

    CRISPR

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

17. Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)

    Pooled Library

    #1000000049

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human genome

    Depositor

    Feng Zhang

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Mouse Chromatin Regulator Library

    Pooled Library

    #200011

    Purpose

    Knockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.

    Depositor

    Bradley Bernstein, Gabriel Griffin, Robert Manguso

    Article

    Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

19. hCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/gene

    Pooled Library

    #83971

    Purpose

    Human CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug Targets

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

20. mTF-CRISPRai

    Pooled Library

    #122131

    Purpose

    Increases transcriptional repression of genes encoding transcription factors in combination with dCas9 repression systems, such as the dCas9-KRAB system.

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only