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We narrowed to 253 results for: Mac

Showing: 1 - 40 of 253 results

  2. Caltech Systemic Capsids

    Type
    Collection
    ... - AAV.CAP-Mac For rats: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular...rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9...Caltech Systemic Capsids PHP.eB PHP.S PHP.V1 MaCPNS1 MaCPNS2 CAP-B10 CAP-B22 AAV9-X1.1 As part of our Viral...Gradinaru MaCPNS1 These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid...Boyden MaCPNS2 These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid...Pubmed 32313222 (Link opens in a new window) MaCPNS1, MaCPNS2: Chen et al., 2022 Pubmed 35643078 (Link opens...NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...

  3. Hot Plasmids February 2024

    Type
    Blog Post
    ...bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed...primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific...rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human...2023). They found the best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier...to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco, Flytzanis, Goeden...2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C.,...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence...terminators, etc) are composed. Fear not – the state machine design space is highly degenerate and often times...Roquet et al., Synthetic recombinase-based state machines in living cells. Science. 353, aad8559 (2016).... recombinase-based state machines...

  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...is magnetic bead-activated cell sorting, or MACS. But MACS usually only allows for sorting based on a ...achieves greater purity of your sorted cell sample than MACS. An effective strategy to sort out your cells of...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    ...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ... cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...al, 2023 AAV.CAP-Mac CNS: neurons (rats, infant green monkey, rhesus macaque); vasculature (adult...Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green monkeys (Chuapoco...caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid, developed and deposited by ...selected from a library screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets...astrocytes (Chuapoco et al., 2023). Additionally, AAV.CAP-Mac has a lower transduction rate than AAV9 in the liver... rhesus macaques (Chen et al., 2022). AAV.MaCPNS2 is slightly more effective than AAV.MaCPNS1 at transducing...rats); CNS neurons (infant rhesus macaques) Mice, rats, rhesus macaques No (mechanism unknown)   Chen...

  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

  12. Optogenetics Guide

    Type
    Guide
    ...rhodopsins Mac Light-activated outward proton pump from Leptosphaeria maculans 540 eMac 3.0 Mac with addition...Leptosphaeria maculans . Mac and its variants allow for inhibition of neurons using blue-green light. Mac Variants...Archaerhodopsin plasmids . Wild-Type Mac Leptosphaeria rhodopsin (Mac) is a blue-green light-activated proton...Variants. Mac variants have been engineered to include enhancements such as: Improved photocurrent amplitude...amplitude Example: eMac3.0 Browse Leptosphaeria plasmids . Glossary of Microbial Opsin Variants Opsin Type...

  13. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic...NaCl to destabilize ionic interactions between macromolecules. The 40% and 25% steps are used to remove contaminants...option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua...

  14. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...receptor 3 and 4 subunit) CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7 KIR2DL1 killer cell immunoglobulin-...prosome, macropain) 26S subunit, ATPase, 3 MGC8487, TBP1 PSMC4 proteasome (prosome, macropain) 26S subunit...prosome, macropain) 26S subunit, non-ATPase, 10 dJ889N15.2, p28 PSMD11 proteasome (prosome, macropain) 26S...prosome, macropain) 26S subunit, non-ATPase, 14 PAD1, POH1, rpn11 PSMD2 proteasome (prosome, macropain) 26S...prosome, macropain) 26S subunit, non-ATPase, 3 P58, RPN3, S3 PSMD4 proteasome (prosome, macropain) 26S subunit...ligand 18 (pulmonary and activation-regulated) AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18 CCL19...

  16. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS1 Cap controlled...pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled...

  18. Control AAV Preps

    Type
    Collection
    ...Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa CMV Dlx EF1a...6, 8, 9, 11, rg*, PHPeB, CAP-B10, CAP-B22, MaCPNS1, MaCPNS2, AAV9-X1.1 Boyden 44332 pZac2.1 gfaABC1D-tdTomato...

  19. Genetic Code Expansion

    Type
    Collection
    ...translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery are ...-L-lysine Bacterial TAG David Liu 104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial...Mammalian TAG Ivana Nikić-Spiegel 182537 pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial TAA Wenshe Liu...

  20. DNA Quantification

    Type
    Protocol
    ...Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure...

  21. Antibody Guide

    Type
    Collection
    ...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...direction based on the emission(s) read by the machine. Controls for cell sorting methods Flow cytometry...overlap to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

  22. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell. 2020 Dec 12. pii: S0092-8674(20...Superecliptic pHluorin pH sensor Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing...cells. Circ Res. 2010 Feb 19;106(3):526-35. Paul Schumacker Redox/ROS Glutathione redox sensors for cytosol...Voltage sensors for use in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based...

  23. AAV Viral Preps

    Type
    Collection
    ...Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida Serotype...

  24. Chemogenetics Plasmids

    Type
    Collection
    ...Activated by Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs...respond specifically to small molecules termed Pharmacologically Selective Effector Molecules (PSEMs). PSAMs...

  25. mTOR Pathway

    Type
    Collection
    ...proliferation, including an abundant supply of macromolecules and energy, as well as the presence of both...PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg...

  27. Brain Armamentarium

    Type
    Collection
    ...promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG...promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG...

  28. Luciferase Plasmid Collection

    Type
    Collection
    ... using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC Firefly Creation of C-terminal ... using Gateway cloning. Use with 174050 Alberto Macho 136010 psi-CHECK3 Firefly, Renilla Insertion of ...

  30. Resolute Plasmid Collection

    Type
    Collection
    ...well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project has received...

  31. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Pubmed PMID: 3325793 Schumacher MA (2012) Bacterial plasmid partition machinery: a minimalist approach...origin of replication and the replication control machinery (Need a refresher on the Origin of Replication...they compete for the same replication control machinery within the cell. Most plasmids encode a negative...through inhibiting translation of replication machinery proteins, or directly inhibit replication by binding...origin of replication and blocking replication machinery (del Solar, 1998). As the copy number of the plasmid... structure that physically blocks replication machinery when plasmid copy number is high (del Solar et...replication of each plasmid using unique replication machinery. However, when plasmids have the same origin of...

  32. Synthetic Biology - Overview

    Type
    Collection
    ...Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological Parts...

  33. Deep Dive: qPCR

    Type
    Blog Post
    ...and most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler,...previously-generated validation data, be aware that different machines, samples, and treatments may affect the assay....running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr chemistry is ... by bubbles in wells, variations in volume, or machine surge errors and allow for well-to-well normalization... likely need to re-optimize protocols for that machine. (Teo, et al, 2002; see paper for more information...information on optimizing for LightCycler machines.) Multiplexing It is possible to multiplex qPCR when using...

  34. Lab Automation at Addgene

    Type
    Blog Post
    ...that you come into the lab wishing there was a machine that could make your life a hundred times easier...off our hands. Good news! There is probably a machine or robot out there that does exactly what you want...lot of time. Enter the Biomek FXP! We wanted a machine that would improve our transformation protocol ...played a factor in our decision to go with this machine, but we also really liked that Beckman was flexible...apart before plating. With this fine piece of machinery we have been able to transform 96 samples in about...lab team. Enter the Liquid Handler!     This machine fits on a table top and can be easily programmed...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...

  35. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions... can also download the software for use on any machine with OpenCL enabled hardware. This OpenCL dependency...genomes), then it is worthwhile buying a dedicated machine. On the offline front, there is a Python script...Pasteur, Paris. You can follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al....

  36. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...code of life. In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty’s experiment debunked the myth that...to stars in space ships and the rise of killer machines that exterminate entire humanity were the two ... technologies rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation... the use of molecular dynamics simulations and machine learning to develop a predictive understanding ...://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod CM, McCarty M (1944) STUDIES ON THE CHEMICAL NATURE...

  37. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...

  38. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin... Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins that are...channelrhodopsin that’s successful in vivo. Using machine-learning to overcome channelrhodopsin design challenges...challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help them construct “designer...

  39. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... passes through the machine’s lasers and gives off a signal read by the machine’s sensors. (Usually this...or unbound antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate ...variations in fluorescent signals, and how the machine reads/interprets the signals, the axis is instead...populations. This shape is called a ‘gate’; it tells the machine that everything inside the gate is one population...

  40. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...
Showing: 1 - 40 of 253 results