We narrowed to 735 results for: RAN-1

...mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature Protocols 2013). For ...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature...and its single guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses...Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu... Cas9 nucleases. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X,...

... edits via homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild-type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...

...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with...Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases (CASTs) combine...tables below for CRISPR transposase plasmids. To learn more about CRISPR transposases and other CRISPR topics...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...combine the large-scale capacity of transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems...lacks nuclease activity, instead recruiting the transposase (comprised of multiple components) to target ...locations in the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs...

...occurs at a much lower efficiency than NHEJ. Figure 1: Overview of NHEJ and HDR following cleavage by Cas9...CRISPR plasmids that create double strand breaks in DNA. CRISPR... functional Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

... cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II bacterial...short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA...Nature . 550(7676):407-410. PMID: 28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu...CRISPR-Cas9. Cell . 167(7):1829-1838. PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz...engineering, with Type V following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain...enChIP) using CRISPR. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Gilbert LA, Horlbeck MA, Adamson...CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol . 35(1):31-34. PMID: 27918548...

...IGHDY1 IGHD1-1 immunoglobulin heavy diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-...IGHV6-1 immunoglobulin heavy variable 6-1 IGHV61, VH IGHV7-4-1 immunoglobulin heavy variable 7-4-1 IGHV7...TAPBPR THBS1 thrombospondin 1 THBS, THBS-1, TSP, TSP-1, TSP1 TRPC4AP transient receptor potential cation...variable 2-8 IGLV28, V1-2 IGLV3-1 immunoglobulin lambda variable 3-1 IGLV31, V2-1 IGLV3-10 immunoglobulin lambda...NCC-1, NCC1, SCYA13, SCYL1 CCL14 chemokine (C-C motif) ligand 14 CC-1, CC-3, CKb1, FLJ16015, HCC-1, HCC... 3 G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3 CCL3L1 chemokine (C-C motif) ligand 3-like 1 464.2, D17S1718...GPD, GpFy, WBCQ1 DEFA1 defensin, alpha 1 DEF1, DEFA2, HNP-1, HP-1, MGC138393, MRS DEFA3 defensin, alpha...

...dependent 1 Francois St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a JEDI-2P none Cre dependent 1 Francois...none Constitutive 1 Loren Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR none Constitutive 1, 9 Loren Looger...dependent 1, 5, 9, rg* GENIE Project 169258 AAV-hSyn-Soma-jGCaMP8f Syn soma-jGCaMP8f none Constitutive 1, 9,...Constitutive 1, 5, 9, rg* Loren Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg...Constitutive 1, 9 Loren Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg* Loren...dependent 1, 9, rg* GENIE Project 169256 AAV-hSyn-Soma-jGCaMP8s Syn soma-jGCaMP8s none Constitutive 1, 9, rg...Constitutive 1, 9, rg* Loren Looger 176752 AAV-CamKIIa-jGCaMP8s-WPRE CamKIIa jGCaMP8s none Constitutive 1, 5, ...

...Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1.Twitch2B.WPRE.SV40 Biosensor Oliver Griesbeck AV-1-50942 50942...Tobias Rose AV-1-PV2723 98929-AAV1 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2724 98931... Looger AV-1-PV2725 98932-AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2816 100835... Kim AV-1-PV2817 100839-AAV1 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2818 ... Kim AV-1-PV2819 100833-AAV1 pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2820 ... Kim AV-1-PV2821 100845-AAV1 pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2822 ...Douglas Kim AV-1-PV2823 100841-AAV1 pAAV.Syn.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2824 100843...

...dependent 1, 2, 5, 8, 9, rg* Brandon Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre dependent 1, 5 Karl...Constitutive 1, 2, 5, 8, 9, 11, rg*, PHP.eB Bryan Roth 50469 pAAV-CaMKIIa-EGFP CaMKIIa EGFP Constitutive 1, 2, ...Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB, PHP.S Edward Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive...Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth 117382 hSyn1-eYFP hSyn eYFP Constitutive 1, 2, 5, 8, 9,...Constitutive 1, 8 Karl Deisseroth 135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory dTomato Constitutive 1, 9, PHP.eB...dependent 1, 2, 5, 9, rg* Karl Deisseroth 28306 pAAV-FLEX-tdTomato CAG tdTomato Cre dependent 1, 2, 5, 8...dependent 1, 2, 5, 8, 9, rg* Bryan Roth 50459 pAAV-hSyn-DIO-mCherry hSyn mCherry Cre dependent 1, 2, 5, ...

...-phase expressed 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated...inhibitor type 1), member 1 PERP TP53 apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide...receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor ... Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1 ATM ATM serine/threonine kinase...kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger and WD repeat domain 2...SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2...Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also known as PMAIP1 p14ARF Cyclin-dependent kinase...

...Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic ... dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein 1 PIK3 Catalytic Subunits PIK3CA...family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 RHEB Ras homolog enriched in ...protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell...guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return to top Resources RAS Pathway...kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription factor Dp TIAM TIAM1 TIAM2 T-cell ...Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear...

...190293 Anti-AMIGO-1 [L86/14] AMIGO-1 Mouse Mouse IgG2a 190294 Anti-AMIGO-1 [L86/2] AMIGO-1 Mouse Mouse IgG2a...ATPase 1 [L60/4R-2b] Copper ATPase 1 Human Mouse IgG2b 206648 Anti-AMIGO-1 [L86/33R-2b] AMIGO-1 Mouse ...channel [L6/60R-1] Slo1 K+ channel Mouse Mouse IgG1 206695 Anti-AMIGO-1 [L86/33R-1] AMIGO-1 Mouse Mouse IgG2a...Anti-Olig1 [N149/25R-1] Olig1 Mouse Mouse IgG1 206702 Anti-Neurexin-1-Beta [N170A/1R-1] Neurexin-1-Beta Human Mouse...222160 Beclin-1 [N248A/15R] Beclin-1 Mouse Mouse IgG2a 222161 Beclin-1 [N248A/22R] Beclin-1 Mouse Mouse ...Mouse Mouse 190534 Neurexin-1-Beta scFv [N170A/1] N170A/1 scFv Neurexin-1-Beta Human Mouse 190535 PSD-...Mouse 206755 IP3 receptor, type 1 scFv [L24/1] L24/1 scFv IP3 receptor, type 1 Rat Mouse 206756 VSP scFv [...

...Cre.SV40 CamKII none 1, 5, 9, rg* James M. Wilson 105537 pENN.AAV.CMVs.Pl.Cre.rBG CMV none 1, 2, 5, 8, 9, rh10... none 1, 5, 8, 9, rg* Esteban Engel , Alexander Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Connie...Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn none 1, 2, 5, ...-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB...mCherry (not a fusion tag) 1, rg* Karl Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Karl Deisseroth...pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Li Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Connie Cepko 183412...pAAV-EF1a-iCreV EF1a none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Hongkui Zeng 140137...

...Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg...Constitutive 1, 5, rg* Edward Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn ChrimsonR tdTomato Constitutive 1, 5, 9...Constitutive 1, 5 Ofer Yizhar 198511 pAAV_hSyn-DIO-PdCO-mScarlet-WPRE Syn PdCO mScarlet Cre dependent 1, 5 Ofer...pAAV-S5E2-ChR2-mCherry nEF ChR2 mCherry Constitutive 1, 9, PHPeB Jordane Dimidschstein 137142 pAAV-nEF-Con...-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre dependent 1, 5, 8, 9, rg* Karl Deisseroth 20298 pAAV-EF1a-double...EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP Cre dependent 1, 5, 9, rg*, PHPeB Karl Deisseroth 26969 pAAV-CaMKIIa-hChR2...pAAV-CaMKIIa-hChR2(H134R)-EYFP CaMKII ChR2/H134R EYFP Constitutive 1, 2, 5, 9 Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R...

...Syn-driven, Cre-dependent Beta-Secretase to be expressed (1:1) with either ATLAS protein. 8 Don Arnold Don’t See...the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru...expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals. 1 Liqun Luo 60658... Cre-dependent, Tet-inducible expression of EYFP. 1 Viviana Gradinaru Tools for Affinity Purification ...synaptophysin-EGFP for labeling of axon terminals. 1 Hongkui Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby... pro-taCasp3 and TEVp for studying cell ablation. 1, 5, 8 Nirao Shah , Jim Wells 124364 pAAV-FLEX-DTR-...

...accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based...the plasma membrane Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff...cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer...Sorting On Transient Cellular Responses Of Genetically-Encoded Sensors. Anal Chem. 2017 Jan 3;89(1):711-719...physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring...of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam Cohen Calcium GCaMP5 genetically encoded...in High-Ca 2+ Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. Teresa Alonso , Javier...

...hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 44362 pAAV-hSyn-DIO-hM4D...hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan Roth 50475 pAAV-hSyn-hM4D(Gi...mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan Roth 154867 pAAV-hSyn-fDIO-...mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9 Bryan Roth 50477 pAAV-CaMKIIa-hM4D(Gi)...mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9 Bryan Roth 75033 pAAV CD68-hM4D(Gi)-mCherry...mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 9 Bryan Roth 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell...

...library as GST-fusion peptides (“Mode #1” expression). The Mode #1 phosphosite library (Pooled Library #111704...References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine to tRNA^Sep with anticodon...standards for quantitative assessments. The Mode #1 phosphosite library can be generated with either phosphoserine...phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 phosphosite library configuration for ...Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also be synthesized using ...iSPI_pSer_Subpool#2 Pooled Library 188526 iSPI_pSer_Subpool#1 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid... #2 Library (14-3-3β) Pooled Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid...

...Communications , 15 (1), 3663. PMID: 38688943 Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9..., 1163–1171. PMID: 23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D..., 17 (5), 471–479. PMID: 32203383 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I....CRISPR-Cas9. Cell , 167 (7), 1829-1838.e9. PMID: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott...Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg...Communications , 14 (1), 6175. PMID: 37794046 Small precision edits Anzalone, A. V., Randolph, P. B., Davis,...

...rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase...Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin D1 Also known as CCND1...RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known ...Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor...protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG...complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...

...EYFP 434 0.41 527 67,000 0.67 4.8 1.5 pmECFP-1 , pmEYFP-1 , pECFP18aaEYFP mCerulean mVenus 433 0.49 527...nm), ε A : Acceptor exctinction coefficient (M -1 cm -1 ), QY A : Acceptor quantum yield, R 0 : Förster...emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator...increased energy transfer through FRET reduces the donor lifetime. FRET is often used to study (1) protein-protein...pBAD , pLSSmOrange-mKate2 LSSmOrange* mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3...Resonance Energy Transfer (FRET) is a process by which energy is non-radiatively transferred from an excited...2.9 mVenus N1 , mKO2-N1 mOrange2 mCherry 549 0.60 610 72,000 0.22 6.1 4.4 mOrange2-N1 , mCherry2-N1 miRFP670...

...AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-1 plasmid... vector for transgene expression under a CMV promoter pAdEasy®-1 - Recombine ...Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies pMKO.1 GFP - Retroviral ...N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 ...

...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [...Cookson 13321 pGEX5X.1 PINK1 WT PINK1 GST tac Parkinson's Mark Cookson 13322 pGEX5X.1 PINK1 KD PINK1 GST... His, V5 EF-1 alpha Parkinson's Mark Cookson 25081 pDEST51-LRRK2-R1441C LRRK2 His, V5 EF-1 alpha Parkinson's... His, V5 EF-1 alpha Parkinson's Mark Cookson 25083 pDEST51-LRRK2-R1441H LRRK2 His, V5 EF-1 alpha Parkinson's...Cookson 29350 pGEX-5X-1-DJ1-WT PARK7 GST tac Parkinson's Mark Cookson 29351 pGEX-5X-1-DJ1-D149A PARK7 GST...Cookson 29352 pGEX-5X-1-DJ1-E64D PARK7 GST tac Parkinson's Mark Cookson 29353 pGEX-5X-1-DJ1-M26I PARK7 GST...

...mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component...Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP ...TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP ...Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related... HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone H2B type 1-J Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF...

...Nucleus CAF-1 mGold2t Francois St-Pierre 231774 pCMV-mGold2s-CAF1-C-10 Nucleus CAF-1 mGold2s Francois St-Pierre...alpha-actinin 1 Focal adhesions alpha-Actinin 1 EGFP Carol Otey 31923 pPaxillin-PSmOrange Focal adhesions...Brown 11908 pEGFP-N1 alpha-actinin 1 Actin filaments alpha-Actinin 1 EGFP Carol Otey 27676 Cortactin-pmCherryC1...Autophagosomes Sequestosome-1 EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI-1 Autophagosomes WIPI1 EGFP ... Golgi, and Plasma membranes CAAX EGFP Lei Lu 14873 GolgiCFP Golgi apparatus eNOS(1-33) CFP Alexandra ...mCherry-ZO1-C-14 Tight junctions Zonula Occludens-1 mCherry* Michael Davidson 55001 mCherry-Beta-Catenin... 30313 pEGFP ZO1 Tight junctions Zonula Occludens-1 EGFP Alan Fanning *Fusions to other FPs can be found...

...pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter pCDH-EF1-copGFP-T2A-Puro 72263 Express copGFP...pCDH-EF1 72266 Express gene of interest from the EF-1 promoter pCDH-CB 72267 Express gene of interest from...72484 Express gene of interest from a truncated EF-1 promoter pCDH-EF1-Luc2-P2A-copGFP 72485 Expresses ...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...

...Small NEW 2 × 100 µL aliquots 0.2 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 10 × 100 µL aliquots 1.0 mL ...to 10 weeks. This breaks down as follows: Request 1–2 days Look for the banner on an eligible plasmid ...place your request. We will send you an email within 1-2 days with a response. Most requests will be approved... we can begin processing your order. MTA Approval 1+ business days, varies by requesting organization ...scientist through an MTA. This process typically takes 1–4 days, depending on your institution. AAV Production...tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...

...endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems, the most abundant type found...bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared to their...still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with ...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

... Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid Vogelstein...production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use in AdEasy™ System Vogelstein...plasmids: A shuttle plasmid (or transfer plasmid) containing the transgene of interest, the ITRs, and homology...packaging capacity, high efficiency in driving strong transient gene expression in both dividing and non-dividing...pShuttle For production of viruses containing transgene under a chosen promoter Vogelstein 16403 pShuttle-CMV...pShuttle-CMV For production of viruses containing transgene under CMV promoter Vogelstein 16404 pAdTrack For...production of GFP-trackable viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV...

...astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts...Protoc Cell Biol. 2018 Jun;79(1):e51. Next generation vectors for transcription factor mediated differentiation... with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human...cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome Human Non-integrating...vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible...peripheral sensory neurons. Nat Neurosci. 2015 Jan;18(1):25-35. Baldwin Fibroblasts iTSCs Lentiviral Mouse... from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral...

...Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells for... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

... orientation of each target site (see Figure 1). Figure 1: Recombination outcomes are determined by target...minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and Flp Recognition Sites. Controlling...target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-, Flp-, or...nature of the DNA rearrangement after recombination (inversion, deletion, or translocation) depends on the...sites are on separate DNA molecules (in trans), a translocation event is generated between the target sites...sites are arranged, recombinases can be used for more than inversion, deletion, or translocation of a gene...evaluating infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid...

... design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference. Created...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols... the double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds...silencing is achieved through the delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme...

...tetracycline and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic... ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger expression...Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR from CMV...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA...tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation domain)...

...rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA and G-protein is delivered to the...with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932 Wickersham IR, Lyon DC, Barnard...glycoprotein (G) gene, which is not required for the transcription or replication of the genome within infected...infected cells, but is required for transynnaptic spread (Wickersham et al., 2007a, Wickersham et al., 2007b...essential G protein is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell... cell using the G-protein, which is supplied in trans . (3) Nascent G-protein coated RAVB is released ...Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons...

...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV5 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV8 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV9 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-AAVrg pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-PHPeB pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

...constitutive transgene expression and EGFP coexpression. Didier Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter... . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin...for hygromycin selection. Bob Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid...divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked...envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging...types of viruses used in research. Lentivirus can transduce both dividing and non-dividing cells without a...

...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

...populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions... as a proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach...interneurons encode fear memory. Nat Neurosci. 23(1):61-74. PubMed (Link opens in a new window) Hafner...Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link opens in a new window) Lazaridis...including dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing... Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image...in a new window) of Flp-recombinase-expressing transgenic mouse and rat lines. The Fenno Lab maintains ...

...Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial...Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli p-azido-l-phenylalanine (pAzF) Bacterial...recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase and target plasmids in genome code...7FTrp) Bacterial TAG Thomas Huber 207639 pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest...and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair instead. For...Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed Badran 209740 pAB228q12...jannaschii Bacterial Ahmed Badran 209741 pAB228r3 Mlum1RS Bacterial Ahmed Badran 209742 pAB228p PylRS M. ...