We narrowed to 621 results for: Emb
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TypeBlog Post...interested in working for this lab and/or company. Remember to proofread your cover letter, and if possible... originally written by Maria Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024...
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New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...produce ultrasound contrast. That is, until earlier members of our lab - the Shapiro Lab - developed Acoustic...student studying bioengineering. They are both members of the Shapiro lab at Caltech. References and... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...https://youtu.be/EHHhiawIdWM Saving salmon, one embryo at a time – A video by LeeAnne Thayer, a Ph.D. ...University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...harboring this mutation are deficient in outer membrane protease VII, which reduces proteolysis of the...pLemo (CamR) Expression of toxic, insoluble, or membrane proteins T7 Express IPTG-inducible expression... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...cells. GFP is big GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter...Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells." Science 296.5569 ... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post... complementary reporter proteins that can only assemble when in close proximity. Split-BioID consists ...consists of a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...empathy, a willingness to listen, and an ability to remember the right stories at the right time. Like many...morefamousquotes.com A good listener absorbs and remembers and makes the teller feel heard. I am not always... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...recommendations. As you optimize your protocol, always remember to include controls to show that your antibodies...sample). When choosing a primary antibody, first, remember that antibodies recognize very specific 3-dimensional... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post... by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several... guest blogger! Daria Shcherbakova is a faculty member at Albert Einstein College of Medicine. Her expertise... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...polymerase II. The EMBO Journal, 23(20), 4051–4060. https://doi.org/10.1038/sj.emboj.7600385 McIntyre, ... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post... Symposium 2015. I personally participated as a member of the Career Paths Panel on the second day of ...people from all backgrounds are truly included as members of their research communities and are treated equitably... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...selection marker, then electroporate it into mouse embryonic stem cells. After selecting for clones with the...the mutation and inject the ES cells into a mouse embryo to create chimeric mice. This laborious process... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression systems...vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525. Find... -
A Control for All Seasons
TypeBlog Post...and equal protein transfer from the gel to the membrane. A loading control is critical for data interpretation.... Always probe the loading control on the same membrane that the target protein was measured on. Flow... -
Antibodies 101: Validation
TypeBlog Post...Show suitability for an intended application - Remember, just because your antibody performs as expected...is just as helpful as knowing when it will. And remember, antibodies are like puppies. Just because an ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...the background. Dyes that can pass through cell membranes can prove useful for live-cell imaging. Experimental...receptors. In Biochimica et Biophysica Acta - Biomembranes (Vol. 1838, Issue 1 PARTA, pp. 15–33). https... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors...PMC3161101. 20. Chang, C.J., et al., Production of embryonic and fetal-like red blood cells from human induced... -
15 Hot Plasmids from 2017
TypeBlog Post...via genetically encoded proteins. PhoCl is a new member of this class that works via a different mechanism...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. These exciting papers ... -
Addgene’s First AAV Data Hub Challenge
TypeBlog Post...answered. Interested? You can learn more here - and remember, submissions are due Nov. 21! ... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog Post...tagged with #DeckTheLab throughout the month of December are eligible to win a: That will warm up the ... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog Post...what we lacked were videos guiding completely new members of the lab or those taking introductory lab courses... -
'Tis the Season to #DeckTheLab
TypeBlog Post...tagged with #DeckTheLab throughout the month of December are eligible to win a hot chocolate party that... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...to Addgene, Aavery! Original post: In December, we introduced you to Blugene’s new virus friend... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog Post...repository in ways that will be most useful to you, the members of our community. ... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog Post...accelerated. We significantly reduced posting on November 20, 2023, and as of today, will stop posting, ... -
Addgene's 20th Anniversary Party
TypeBlog Post...! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on the welcoming committee... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog Post...Unearthed Comics Feeling any better yet? If not, just remember that the next holiday break is just a few weeks... -
Twenty Years of Sharing Science!
TypeBlog Post...across 55 countries. Our first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.... -
The Codon Guide to Synthetic Biology
TypeBlog Post...gene-editing, the foundations of synthetic biology, DNA assembly, and various applications. I think these papers... -
Expanded Access to Rabies Plasmids
TypeBlog Post...sequences from rabies and all other lyssa genus members to the specified international institution. Requesters... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post..., who added to our career advice collection. Embracing Serendipity Nicolas Mathis, who shared information... -
Bringing Renewable Energy to Addgene
TypeBlog Post...equipment on a daily basis. By the beginning of September all will be powered by 100% renewable electricity... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog Post...We recently sat down with Addgene Board Member, depositor, and Harvard Medical School researcher Connie... -
Summer Fun at Addgene!
TypeBlog Post...this summer! Summer SciComm Series From June-September, we’ll be running a Summer Science Communication... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog Post...University of Edinburgh - synthetic biology, DNA assembly methods, and engineering cellulose degradation... -
Editor's Choice, October 2016
TypeBlog Post...Increased Postdoc Wages I hope you all have a great November! Tyler J. Ford ... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog Post... for Benoit to interview Addgene Advisory Board Member and lead researcher at Imperial College London,... -
Fueled by Coffee at #SfN14
TypeBlog Post...you presenting, working, and networking at SfN, remember there are only 2 days left for you to make the... -
A Scientist's Guide to Ebola
TypeBlog Post...speculation, we thought we’d do a little digging and assemble a rough guide to the science of Ebola and related...