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We narrowed to 522 results for: plasmid dna

Showing: 401 - 440 of 522 results

  1. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... be used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies... target protein. However, small mutations in the DNA of the hybridoma can occur, known as genetic drift...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...protein (Wang et al., 2013). These proteins are also plasmid-derived. However, a disadvantage to ScFv is their...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common internal control ... as well as tools for developing and producing plasmid-based antibodies - and we’re always growing our...

  2. Serotype Testing AAV

    Type
    Collection
    ...virus associated DNA (vdna), which is a sample of the purified DNA from the original plasmid that was used...AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct ...AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...details, see our FAQ on What is virus associated DNA, and why do I have to order it? Don’t See What You...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the next...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... FRET-based biosensors, and interactions between DNA and proteins. FRET, in theory, is an easy technique...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent... fusion protein with your gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells' genomes... ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources Page...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    ...self-complementing structure (scAAV) (7). As single stranded DNA viruses, AAVs normally require second strand synthesis... promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001): 1248. PubMed...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  7. Viral Vectors

    Type
    Collection
    ...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate... this genetic information to cells. Adapting plasmid DNA for use with virus-mediated delivery has many...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...

  8. KLF Research Plasmids

    Type
    Collection
    ...of KLF plasmids and learn more about the scientists who have created and shared these plasmids....Plasmid Collections KLF Plasmids Plasmids for KLF Research KLF Depositing Labs Juan Belmonte Linzhao Cheng...information on available plasmids, references, and links. KLF1 KLF2 KLF4 KLF5 KLF6 KLF Plasmids Browse Addgene'...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...-like factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles...which bind to CACCC elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression...shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute...

  9. TALEN Expression Vectors

    Type
    Collection
    ...Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids This page lists plasmid vectors into ...into which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH... yield TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details of...with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit . Vectors...domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI...

  10. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...any contaminating plasmid DNA carried over from the production process (DNase does not penetrate the virion... calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation Size...Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free DNase 10X DNase buffer Nuclease-free water...better results when the plasmid is linearized. Treat the purified AAV samples with DNase I to eliminate any...

  13. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ... the Doudna Lab . Browse, sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR... CRISPR Nick CRISPR Plasmids: Nick Browse...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...the tables below for CRISPR prime editing plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...prime editor plasmids for (search and replace) genome editing. CRISPR...

  15. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ...by the Doudna Lab . Browse, sort, or search the tables below for CRISPR RNA targeting plasmids. Plasmids...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria, once they have recognized and cleaved...contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity to the spacer ...

  16. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...the tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... by a particular gRNA. In the enChIP (engineered DNA-binding molecule-mediated ChIP) system, catalytically...catalytically inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused ...

  17. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...in the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should be added to...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance... Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...CRISPR plasmids that have been designed for use in zebrafish. CRISPR... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...

  19. CRISPR Plasmids - Parasites

    Type
    Collection
    ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids: Parasites...Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...development. This collection brings together some of the plasmids that have been used in these efforts. The content...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss ...

  20. Rett Syndrome

    Type
    Collection
    ...CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

  21. AAV Packaged on Request

    Type
    Collection
    ...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene... for the transfer plasmid, including legal approval and an implementation letter DNA amplification Viral...applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter ...viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...

  22. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...of two targets independently. The RNA-programmed DNA-binding protein dCas9 is expressed along a CRISPR...expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one...18, or 20 matching nucleotides. Together, these plasmids allow to explore the whole range of expression...amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible... One-Step cloning and chromosomal integration of DNA. ACS Synthetic Biology, 2(9), 537–541. DOI: 10.1021...

  23. Ligation Independent Cloning

    Type
    Protocol
    ...Eluted DNA 10-50 ng/μl 1 dGTP (100mM) 2.5 mM 2 DTT (100 mM) 5 mM 1 BSA (10 μg/μl) 0.25 μg/μl 1 T4 DNA polymerase... does not provide enough stability to hold the plasmid together through the transformation/replication...of its dual polymerase/exonuclease functions, T4 DNA polymerase can create overhangs of varying length...Vector Overhangs Treat the linearized vector with T4 DNA polymerase to "chew back" the free 3' ends, following...

  24. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid Calarco... need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912...elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids that should be added to...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...CRISPR plasmids that have been designed for use in C. elegans. CRISPR...

  25. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...essential-but-unlauded role in the identification of DNA’s double helix. Perhaps to the dismay of middle-aged...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up in this...

  28. Retrovirus Plasmids

    Type
    Collection
    ... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

  29. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...use the chromatin IP (ChIP) technique to identify DNA binding proteins involved in histone modification...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

  31. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable for...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7...Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag...Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid CLIP-tag...Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid ACP/MCP-tag...

  32. Antibody Guide

    Type
    Collection
    ...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays... uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the complex...suitable for DNA:histone modifier interactions, which have strong binding between the protein and DNA. Some ...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...

  33. Promega Plasmid Collection

    Type
    Collection
    ...Promega Plasmid Collection Promega Plasmid Collection Ordering & Availability All Plasmids Plasmid Technology...agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25 ng/µL) All plasmids in the collection are available...our help center . Plasmid Collection Browse, sort, or search the table below of plasmids in the collection...HaloTag", "BiT", or other terms. ID Plasmid Description Industry Plasmid Technology Overview This collection...Browse Addgene's Luciferase Plasmid Collection Explore other Promega plasmids available at Addgene Find ...operational complexity. This unique collection contains plasmids developed by Promega scientists and designed to...HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids from the Promega collection are available in two...

  34. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system...

  35. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ... non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the...on Addgene.org Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries...fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in genome-wide...function, i.e. sites that lack open chromatin marks, DNase hypersensitivity, or are in an enhancer, transcription...control for the effects of guide expression and dsDNA breaks. Figure 2: Non-targeting vs. safe-targeting...for the inherent effects, or background noise, of dsDNA breaks caused by CRISPR. See supplemental figure...

  36. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction This protocol... plate and select transformed cells containing plasmids differing in their resistance genes, as one does...or other antibiotic) E. coli transformed with a plasmid containing the carbenicillin (amp) resistance gene...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...transform DH5α E. coli cells by heatshock with the plasmid of interest. See our transformation page for a ...

  37. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...developed a self-labeling fusion tag based on the human DNA repair protein AGT that gets covalently linked to...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...

  38. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids for RNA editing...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...

  39. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize...Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in... sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

  40. TALEN Engineering

    Type
    Collection
    ... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ...arrays on a single architectural framework and the DNAs produced by all three methods are identical in sequence... simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This ...REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to...Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and...
Showing: 401 - 440 of 522 results