... Plasmid Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent...fluorescent. A variety of organic dyes and fluorescent proteins have been developed for use as donor and acceptor...intramolecular or unimolecular FRET). Fluorescent proteins can be used as donor and/or acceptor molecules...suitable for creating individual fluorescently tagged proteins to study protein-protein interactions and a series...laboratory. Empty Vectors Encoding Fluorescent Proteins for FRET The following plasmids can be used to...brightness standard consisting of two Venus fluorescent proteins connected via a 5 amino acid linker V17V Anisotropy...brightness standard consisting of two Venus fluorescent proteins connected via a 17 amino acid linker V32V Anisotropy...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...Marker PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base... been designed for use in Drosophila and other insects. CRISPR... been designed for use in Drosophila and other insects. Cut Fully functional CRISPR/Cas enzymes will introduce...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...

... TAG Edward Lemke Browse Strains The table below highlights bacteria strains that have been modified to...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...Rinehart Lab reagents tRNA/Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for ...genetic code can help us understand and evolve proteins for various purposes from therapeutics to biopolymers...phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic code expansion without buildup of...DE3) ΔserC serC gene knocked out; for expressing proteins containing site-specific non-hydrolyzable phosphoserine...and trpT genes). Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS...

...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically...the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime...

...Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization More...as these well-characterized proteins. General Structures The cell contains a variety of organelles and...role in secreting proteins from the cell to the extracellular environment. Proteins are first translated...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection FRET Biosensors...has assembled a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular...FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...

...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base ...the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate...activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere...

...Genetics 2014 Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...end-joining), an alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor...'s lab for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap...template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in...

...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178...with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ... ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962...and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells ...

...(deletions or small insertions) or (B) span the genome-insert boundary (insertions only). The PCR product...dual) gRNA targeting 5′ exon or essential protein domains High-fidelity Cas enzymes increase specificity.... gene, such as generating a point mutation or inserting a tag Cas9 (or Cas9 nickase); Base editor Single...using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer vector and used to generate...target exons that code for known essential protein domains. The benefit of this approach is that even non-... function when they occur in important protein domains. For gene editing experiments using HDR, it is ...targeting oligos are synthesized, annealed, and inserted into plasmids containing the gRNA scaffold using...

...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection....Plasmids Pooled Libraries Feedback Solute carrier proteins (SLCs) are the largest family of transporters ...associations. Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...Superti-Furga CRISPR knockout library targeting human SLC proteins. Mouse SLC Knockout Library 141436 Superti-Furga...

...of interest, a medium copy RSF1030 origins or a low copy p15a origins should be used. Find further details...system to make singly or multiply phosphorylated proteins (Pirman et al., Nat Comm 2015). Improved phosphoprotein...improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ...other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express the...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...phosphosite library with four different phosphobinding domains (14-3-3β, 14-3-3σ, the WW2 domain of NEDD4, and...68307 ) to generate the phosphosites with serine instead of phosphoserine (i.e. a “phosphorylatable” phosphosite...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including Markers...Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...with the mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. ...with concomitant expression of aggregation-prone proteins with the iPSC toolbox . Lam et al. bioRxiv. 2022...A foundation dedicated to finding a cure for Parkinson's disease through an aggressively funded research...

... Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research ...SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...

...Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For multicistronic...different fluorescent tags for you to create fusion proteins with your gene of interest. Special... Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set of colors. Addgene...different fluorescent tags for you to create fusion proteins with your gene of interest. These are organized... at high concentration, so this is noted here. Proteins that are derived from GFP and contain the A206K...information on the properties of many of these proteins, see: Cranfill et al. , Nature Methods 2016 Jul...

...nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations using...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter ...edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate for another...small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding exonuclease protection...enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide...

...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids... vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids ...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids for building homologous repair...Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes...Caenorhabditis Genetics Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing...

...protocol can easily be adapted for other types of proteins.    If this video is helpful, you can find our...

...into the host genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral...Type Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev; can be used with 3rd generation transfer ...Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be used with 3rd generation transfer... encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and...plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and...plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used ...envelope plasmid Kasparov 22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd ...

...Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden...environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain...terminators, repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab...Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning ...ethical synthetic biology JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering Research...

...the Lab" shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation...

...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal D omain...or bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator...that can be differentiated into neurons Coriell Institute: Patient-specific lines (Link opens in a new window...Repositories (Link opens in a new window) Coriell Institute - cell line repository, including patient-derived...Additional Resources (Link opens in a new window) BrainSpan Atlas of the Developing Human Brain - includes... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

...announce the launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll ...

...our videos will also be shared on our @addgene Instagram account, or you can watch the full animation on...

...causing him some difficulty… To give him some inspiration, we’d love to see how you’re decorating your ...

...synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA...sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve...Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514 pOpen-... to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase...Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506 pOpen-Eco31I Eco31I... Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia... to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol...

...of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction of total...relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total.... Three to five days later, cell cultures are inspected for sterility. Transducibility Some viral vectors...virus from Addgene, read our detailed recipient instructions for next steps. Plan an experiment using our...to amplify a portion of the intended lentiviral insert. During this cultivation, cells are routinely checked...

... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource..., and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's Disease research. This collection is part of ...search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

... almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often used...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (comprised of multiple...TniQ). Type I CASTs can result in bidirectional insertions and may not be suitable for applications where...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...specific biological processes. Addgene’s collection contains two main classes of chemogenetic receptors: Designer...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you have suggestions...

...fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are...collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive...promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources...Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb 19;9(1):2234...

...series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous system, and...

...CRISPR, Addgene gave to me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed integrases...

...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...

...recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences into the open reading...recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase...intersectional experimental design. The Deisseroth Lab maintains a Standard Operating Procedure (Link opens in ...transgenic mouse and rat lines. The Fenno Lab maintains a public folder (Link opens in a new window) of...additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies... Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock... Cre Yes Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock...

...day of CRISPR, Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to ...

...Deck The Lab contest submissions on Twitter and Instagram and we’re excited to share these creative entries...

...for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids...are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools ...somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells over time... lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic loci in... zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene Blog Posts...Center (ZIRC) - A US-based repository for zebrafish strains and materials and information about zebrafish research...Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish...

...also be sharing our conference experience on X, Instagram, and Bluesky.   Finally, if you're looking to ...

...methyltransferase INSR Insulin receptor INSRR Insulin receptor-related receptor IRS IRS1 IRS2 Insulin receptor ...for the gene, or browse the gene list below. For proteins with multiple subunits or isoforms, individual.... The RAS Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream...

...genus members to the specified international institution. Requesters will need to provide a brief description...

...Your Agarose Gel Other Resources Read about Gel Stains on the BitesizeBio Blog ...

...video protocols.  These feature step-by-step instructions to navigate various lab processes.  Our most...

...antibody deposit (the Integrin collection) from the Institute for Protein Innovation. An Integrin Antibody ...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker...elegans ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you...

...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...Monomer not a Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use...more about the many applications of fluorescent proteins (FPs) and to find the plasmids that are available.... Regulate Biological Activity Use fluorescent proteins to modulate biological activity, like transcription...consisting of ORFs tagged with a variety of fluorescent proteins, as well as over 100 empty backbones. Fluorescent...Jin Zhang Lab at UCSD Properties of Fluorescent Proteins from Kurt Thorn and UCSF Molecular Expressions...

...American woman whose cervical cancer cells became a mainstain of biological study without her or her family'...

... Any Data repository   YCharOS Human proteins Immunoblot (western blot), IP, and IF. Data...