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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 321 - 340 of 960 results

  2. Distribution to Industry

    Type
    Collection
    ... Recipient Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist...Agreements on our Technology Transfer Information page for more information. To learn more about Addgene’s MTA... Plasmids Browse Antibodies Ordering and MTA Information Featured Collections COVID-19 SARS-COV-2, ACE2...account. Please see our Help Center for more information on pricing, payment, and billing. Industry Material...

  3. Retrovirus Plasmids

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...mutagenesis). Read our γ-Retrovirus Guide for more information about: γ-Retroviral packaging systems Frequently...

  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...translational research may require the rigor of both forms of off-target analysis in order to meet clinical...Biased Genotyping Options Technique Sequence Information Detection Limit Advantages Disadvantages Mismatch...experiment is targeted amplicon sequencing. The information from targeted amplicon sequencing is highly sensitive...link genotype and phenotype. It is also key to perform these validations as translational researchers ... NGS-based methods provide the most complete information profile regarding putative off-target sites including...disease, but to treat patients, as well. For more information and a detailed overview of the aforementioned...the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse ...

  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression of target protein as N-terminal fusion form with Sumo tag Trx (thioredoxin) Protein purification...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...delivery. Visit our viral vector page for more information. Below are some common delivery methods. Delivery...plasmid View our Adenovirus Plasmids page for more information Genome Modifications, Reporter Assays, mRNA Regulation... Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral expression ...Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids with G418 selection Find nematode ...ubiquitin promoter for Agrobacterium-mediated transformation Blasticidin Mammalian pLX304 - 3rd gen lentiviral...

  8. Microbiology Resources

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... recreate the diseases they cause. The standard format for providing individual plasmids is a bacterial...CRISPR Resources : Find essential background information on using CRISPR in your lab, specific CRISPR ...and gene regulation to sensing and signaling, to perform your synbio experiment. Synthetic Biology Assembly...Bacterial Expression Systems : Find plasmids to perform your experiment in a particular bacterial expression...

  9. Worm Expression Resources

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...self-excising drug selection cassette (SEC). More information regarding this kit can be found at the Goldstein...can be found on the Jorgensen Lab page. More information on miniMos and MosSCI plasmids can be found on...community with accurate, current, accessible information concerning the genetics, genomics and biology...

  10. mTOR Pathway

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each gene page...plasmids for the gene. For components with multiple isoforms or subunits, individual links to each gene page...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...to prevent RNA degradation For more in-depth information on these PE tools, check out our prime editing...

  12. Chemogenetics AAV Preps

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...Plasmids and Chemogenetics Guide pages for more information about this field. Receptor hM3D(Gq) DREADD rM3D...

  13. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... AAV production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral...that encode the AAV genome (i.e., the genetic information that will be contained in the virion). Additional...

  14. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...Department of Astronomy. She studies how planets form around young stars and the instruments we use to...scientist participation in government, evidence to inform policies, a protected place to work, funding for...hyper-competitive environment where getting hired hinges on performing near miracles. When evidence does not affirm ...evidence-based research is vital in combating misinformation and propaganda that limits access to full reproductive...

  15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...The undigested (-, lane 1) DNA duplex and those formed from control (GFP gRNA-targeted) BRAF sites (lanes...what I think are the main tips for each step of performing and analyzing a gene edit using Addgene’s lentiviral...lentiCas9-blast. Anti-Cas9 Western blotting was performed on whole-cell extracts from stable pools of A549...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...should appear less green. Note that there won’t be uniform editing across all the cells in the population,...techniques and how quick and easy it is to share information and regents, it’s exciting to think that no matter...

  17. Plant Plasmids and Resources

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...design of synthetic operons for chloroplast transformation and metabolic engineering in plants (including...set of functional parts for assembling plant transformation vectors - Daniel Voytas. Transcription Factors...Agrobacterium-mediated transient expression or plant transformation - Silin Zhong. RNAi The following collections...Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology: New Tools...thaliana and related species. The Arabidopsis Information Resource (TAIR) (Link opens in a new window) ...

  18. Lentiviral Prep Service

    Type
    Collection
    ...see available in viral format by filling out our Viral Service Suggestion Form (Link opens in a new window...Browse constructs currently available in lentiviral format. Viral...involved in specific biological processes. For more information, see our Guide to Plasmid Pooled Libraries . ... plasmids targeting specific genes. For more information on CRISPR plasmids and function, see our CRISPR...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer...droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence...repository for zebrafish strains and materials and information about zebrafish research. China Zebrafish Resource...zebrafish-specific gene expression database that provides information about when genes are active in developing embryos...
Showing: 321 - 340 of 960 results