We narrowed to 361 results for: tac

... for ligation of inserts to vectors than for an intact control plasmid. Incubate the competent cell/DNA...

...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...

...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.1% Crystal Violet, Ward’...

...observations. Some microscopes have digital cameras attached to them that allow you to capture images directly...

...place the tip near the bottom of and gently in contact with the container (capillary action between the...

...below the 60–40% interface with an 18 ga needle attached to a 10 mL syringe. The bevel of the needle should...

...careful, the pellet is harder to see and less well attached to the tube after the 70% ethanol wash. You can...

...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...

...gel easier. Cover the electrophoresis chamber and attach to a power supply. Run the gel at 150 V for 1 h...

...using a Gigaprep can be used to obtain sufficient intact pooled library even in the face of a recombined...

...channel variants from other species include: GtACR1 and GtACR2 (from the cryptophyte Guillardia theta ). ... mutation C128A (derived from C1C2 chimera) 475 GtACR Chloride-conducting channel from Guillardia theta...that bind to the LOV domain only in the dark. attaching only one member of the Zdk/LOV2 pair to the target...

...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...direct ELISAs, an antigen or protein of interest is attached to the well of a 96-well plate. A conjugated primary...recognize the protein in its native conformation. Attachment of the antibody to the beads is typically done...are assayed and the extracellular matrix remains intact. The signaling molecule may be a fluorophore or...

...of recombinant DNA technology has overcome this obstacle by enabling the preparation of more manageable...barcode, and amplified. These DNA fragments are attached to a glass slide so that different fragments of..., or templates, are spatially separated. These attached DNA templates are then amplified again, producing...

...inhibition Tung et al., 2015 iLMO: GeNL_SS- hGtACR1 GeNL_SS hGtACR1 CFz Cl - influx Neuronal inhibition Ikefuama...

...different plasmids, the viral genome remains largely intact. First-generation plasmids include (Figure 2): ...with most experiments, infection risks occur with contact to mucous membranes or broken skin. Needle sticks...

...elements are needed. One strategy to overcome this obstacle, has been to use smaller Cas9 orthologs, such ...with most experiments, infection risks occur with contact to mucous membranes or broken skin. Needle sticks...

...tryptophan Promoter from E. coli tryptophan operon Ptac Constitutive Hybrid promoter of lac and trp; constitutive...

...amplified with specific Gateway attB1 and attB2 sites attached to the 5’ and 3’ ends of the DNA sequence. This...

...with most experiments, infection risks occur with contact to mucous membranes or broken skin. Needle sticks...

...with most experiments, infection risks occur with contact to mucous membranes or broken skin. The risk of...