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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 362 results for: tac

Showing: 341 - 360 of 362 results

  1. Fluorescence Titering Assay

    Type
    Protocol
    ...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...

  3. Lentivirus Production

    Type
    Protocol
    ...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...

  4. Colony Formation Titering Assay

    Type
    Protocol
    ...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.1% Crystal Violet, Ward’...

  5. Using a Light Microscope Protocol

    Type
    Protocol
    ...observations. Some microscopes have digital cameras attached to them that allow you to capture images directly...

  6. Pipetting Protocol

    Type
    Protocol
    ...place the tip near the bottom of and gently in contact with the container (capillary action between the...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...using a Gigaprep can be used to obtain sufficient intact pooled library even in the face of a recombined...

  12. Optogenetics Guide

    Type
    Guide
    ...channel variants from other species include: GtACR1 and GtACR2 (from the cryptophyte Guillardia theta ). ... mutation C128A (derived from C1C2 chimera) 475 GtACR Chloride-conducting channel from Guillardia theta...that bind to the LOV domain only in the dark. attaching only one member of the Zdk/LOV2 pair to the target...

  13. Antibody Guide

    Type
    Guide
    ...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...direct ELISAs, an antigen or protein of interest is attached to the well of a 96-well plate. A conjugated primary...recognize the protein in its native conformation. Attachment of the antibody to the beads is typically done...are assayed and the extracellular matrix remains intact. The signaling molecule may be a fluorophore or...

  14. Molecular Biology Reference

    Type
    Guide
    ...purposes of isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant ...barcode, and amplified. These DNA fragments are attached to a glass slide so that different fragments of...are spatially separated from each other. These attached DNA templates are then amplified again producing...

  15. Chemogenetics Guide

    Type
    Guide
    ...inhibition Tung et al., 2015 iLMO: GeNL_SS- hGtACR1 GeNL_SS hGtACR1 CFz Cl - influx Neuronal inhibition Ikefuama...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...different plasmids, the viral genome remains largely intact. First-generation plasmids include (Figure 2): ...with most experiments, infection risks occur with contact to mucous membranes or broken skin. Needle sticks...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...elements are needed. One strategy to overcome this obstacle, has been to use smaller Cas9 orthologs, such ...with most experiments, infection risks occur with contact to mucous membranes or broken skin. Needle sticks...

  18. Promoters

    Type
    Guide
    ...tryptophan Promoter from E. coli tryptophan operon Ptac Regulated like the lac promoter Hybrid promoter ...

  19. Cloning

    Type
    Guide
    ...amplified with specific Gateway attB1 and attB2 sites attached to the 5’ and 3’ ends of DNA sequence. This fragment...

  20. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...with most experiments, infection risks occur with contact to mucous membranes or broken skin. Needle sticks...
Showing: 341 - 360 of 362 results