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We narrowed to 1,053 results for: FER

Showing: 31 - 60 of 1053 results

  1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...supporting in-person conference attendance, the award now supports virtual conference attendance. We also...Michael Davidson and Roger Tsien Commemorative Conference Awards. Congratulations to the five winners! ...awards help early career researchers attend a conference of their choice while honoring the late Michael...and highly interactive environment that Janelia offers,” says Aggarwal.In addition to the biosensors, ...hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase (MPST) which catalyzes the desulfuration of...an enzyme-bound hydropersulfide that is then transferred to thiol-containing acceptors. These hydropersulfides...redox modification, the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its ...

  2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...scientific network We refer to the process of mentoring within a bin as Horizontal Transfer. This comes from...from horizontal gene transfer, which occurs when bacteria of different evolutionary lineages share genes...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...other and what to do.  Saved by the Tweet - The conference hashtag I had, however, started a Twitter account...like anyone at a meeting who felt isolated at a conference for any reason was a Singleton. Figure ...

  3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...is redifferentiated to a different cell type. Transdifferentiation In this process, differentiated adult...reversal of a differentiated cell type to an undifferentiated state and then redifferentiation into the cell...variety of plasmids for differentiation here. Transdifferentiation Dedifferentiation to an intermediate pluripotent...ability to convert a differentiated cell to a different cell type. While transdifferentiation is suited for ...ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which then proliferates and is... are converted to cells of a different lineage, without dedifferentiating into a pluripotent stage. Cellular...pluripotent state. Redifferentiation - Conversion of the pluripotent cells into differentiated cells of choice...

  5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...comes the need for new conference room names. The Addgenie’s favorite conference room naming scheme? Gene...largest conference room at Addgene is named Sonic Hedgehog but this is not a direct reference to the video...community. Not surprising, this is where most of our conference room names originated from. Let’s take a look...Groucho, a transcriptional co-repressor The first conference room, that is actually used as a break room for...diffuses down a concentration gradient exerting a different effect on the developing embryo depending on its...Surprisingly, Sleeping Beauty is not only a princess who suffered a terrible fate when she pricked her finger on...required for spermatogenesis One of our smaller conference rooms is named Cookie Monster. cookie monster...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...IFNA10 interferon, alpha 10 MGC119878, MGC119879 IFNA13 interferon, alpha 13 - IFNA14 interferon, alpha...IFNA16 interferon, alpha 16 - IFNA17 interferon, alpha 17 IFNA, INFA, LEIF2C1 IFNA2 interferon, alpha ...MGC142200 IFNA5 interferon, alpha 5 INFA5 IFNA6 interferon, alpha 6 - IFNA7 interferon, alpha 7 IFNA-J IFNA8...

  7. Plasmids for Stem Cell Research

    Type
    Collection
    ...cells are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...Research Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids...can be directly differentiated into specific somatic stem cells or fully differentiated cell types. Browse...collection of Differentiation Plasmids below. Alternatively, it is also possible to directly differentiate one ...one differentiated somatic cell into another via transdifferentiation. This method of cell lineage conversion... of Transdifferentiation Plasmids below. Many of the techniques for reprogramming, differentiation, and... , SOX2 , MYC , KLF4 , LIN28 . Differentiation and Transdifferentiation Plasmids Browse the table below...

  8. Antibody Guide

    Type
    Collection
    ... dates. Buffers Buffers are a concern for conjugating and signaling molecules, as some buffer components...heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and ...removed from the buffer before the reaction. However, it can be added back to buffers after the conjugation...of colors activated by different wavelengths, allowing for easy differentiation between multiple antibodies... In some cases, it may be preferable to buy small aliquots of many different antibodies and do batch validation...an antigen, while the specific binding site is referred to as an epitope. Production Antibodies are typically...typically produced in one of three different methods: in animals, in cultured immune cells, or through...

  9. Validated gRNA Sequences

    Type
    Collection
    ... 68897 interfere S. pyogenes 26918244 Lu NanoLuc synthetic GACAGAACGATGCGCTGAAT 68898 interfere S. pyogenes... 68895 interfere S. pyogenes 26918244 Lu NanoLuc synthetic TTGATCCAAATTATAACCCG 68896 interfere S. pyogenes...AAACAAAACCTCATCAGGCA 68900 interfere S. pyogenes 26918244 Lu PcfiA GAAGCTCACTCCTTAGCACG 68899 interfere S. pyogenes ...74066 interfere S. pyogenes 26829286 Lu pgi C. glutamicum TTGCCTGGAAGTTTGAGTAATGAT 74067 interfere S. pyogenes...74068 interfere S. pyogenes 26829286 Lu pck C. glutamicum TCCAGTTCAGCAGTTCCTTATTCT 74069 interfere S. pyogenes...74070 interfere S. pyogenes 26829286 Lu pyk C. glutamicum ATTCCATCTGCACTAGCCACCGCT 74072 interfere S. pyogenes.... thetaiotaomicron GAAAATGGGGTGTATCCTGC 68892 interfere S. pyogenes 26918244 Lu BT1854 B. thetaiotaomicron...

  10. Plan Your Experiment

    Type
    Collection
    ...many different Cas proteins available, and researchers can adapt these experiments for different organisms...Desired Genetic Manipulation Different genetic manipulations require different CRISPR components. Selecting...editor variants often have different optimal editing windows. Be sure to refer to the literature associated...discrepancies with the reference sequence you used for gRNA design. Addgene offers many plasmids containing...Activation/interference Needs good primer design Western blot Knockouts Activation/interference Needs a ...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S...have expanded the CRISPR toolbox to include many different types of edits, including point mutations, base...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson 83441 pSOD1E133insTT-AcGFP1...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...Suzanne Pfeffer 186013 pCMV Flag-LRRK2 K17A/K18A/R1441G LRRK2 Flag CMV Parkinson's Suzanne Pfeffer 186016...

  12. Promega Plasmid Collection

    Type
    Collection
    ...nonprofit, Addgene offers expert support, educational resources, and efficient material transfer solutions that...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...experimental workflows. The collection contains luciferase reporter vectors for sensitive gene expression...bacteria as an agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25 ng/µL) All plasmids in the collection..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other...

  13. CRISPR Guide

    Type
    Collection
    ...Alternatives Resources Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview...techniques . Collectively, these techniques are referred to as CRISPR ( C lustered R egularly I nterspaced...measure of fidelity, so sometimes they will be referred to as increased fidelity enzymes. hfCas9s were...pyrimidine-to-pyrimidine) Base transversions - edit to different classification of nucleotide base (purine-to-pyrimidine...opposite strands Prime editing and base editing both offer high efficiency and low indel formation compared...genetic changes, including moving entire genes to different locations, by combining other gene editing mechanisms...DNA-targeting of CRISPR-Cas systems. You may also see them referred to as INTEGRATE ( I nsertion of T ransposable ...

  14. Lentivirus Plasmids

    Type
    Collection
    ...Addgene lentiviral packaging, envelope, and transfer plasmids...Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...encodes the viral genome (typically called the transfer plasmid ). This table lists common lentiviral ... Contains Rev; can be used with 3rd generation transfer plasmids along with additional packaging components...Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev Trono...and Tat; can be used with 2nd and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging...and Tat; can be used with 2nd and 3rd generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... CRISPR References & Information Genomic Deletions in Mammalian ...elements in mammalian cell lines. Protocol For referenced Tables and Figures, please access the manuscript...(100 μM; see step 1.4 ), 1.0 μl 10x T4 Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase...μM; see step 3.1.4 ), 5.0 μl restriction enzyme buffer (10x), 4.0 μl (20 U) BbsI restriction enzyme (5,000...successfully adapted to numerous cell types using preferred culture conditions and transfection strategies... before attempting genome editing. Immediately transfer solution from cuvette into 1 ml of culture media...Minimize the time between electroporation and transferring the solution into media to enhance cell viability...

  16. Open Enzyme Collection

    Type
    Collection
    ...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...Plasmids BIOFAB Plasmid Set Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning ...pOpen-NcoIM M.NcoI DNA methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red...

  17. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-...mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...Library CRISPR page for more information. Full references are below. Return to top Cas9 SAM transcriptional...The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human SAM library...Swiech et al., Nature Biotechnology 2014). Full references are below. Available plasmids are described below...The vector can be digested using BsaI ( This is different from BbsI, which is used in the SpCas9 plasmid...with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin mouse can be purchased...

  19. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this ... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene...

  20. Mammalian RNAi Tools

    Type
    Collection
    ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur via transfection or ...using viral vectors. Lentiviral vectors are a preferred method for long-term gene silencing. Once the ...below. Figure 1: Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids ...pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins...J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746...

  21. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...for a different analyte, a sensor for gene-specific activity, or for popular biosensors in different expression...You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics...protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of fluorescent proteins...the articles linked below include variants with different binding affinities or negative-control constructs...environmental factors and the sensor behavior may differ from the original characterization (e.g. in vitro...the plasmids associated with the article. We also offer ready-to-use AAV preparations of select plasmids...Cre-dependent) Cell-specific restoration of stimulus preference after monocular deprivation in the visual cortex...

  22. Antibody Production

    Type
    Collection
    ...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible...columns. Formulation Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal... cells and will interfere with conjugation. For applications such as these, the buffer can be removed ...webpage. Antibody preparations developed at IPI may differ from Addgene antibodies in “Production & Usage”...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody ...

  23. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...vector, but their function (cut, nick, activate, interfere, etc.) is limited to that of the Cas protein present...have plasmids with Cas9 that can cut, activate, interfere, or nick. Selection , such as Puromycin or EGFP...pyogenes EGFP Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral BfuAI none S. pyogenes...Joung pCRISPathBrick 65006 Bacteria BsaI yes, interfere S. pyogenes Koffas BPK764 65767 Bacteria BsaI ...pyogenes Joung MSP712 65768 Bacteria BsaI yes, interfere S. pyogenes Joung sgRNA with U6 promoter 48962...pyogenes Bar Chen pBUN6I11 50580 Plant BsaI yes, interfere S. pyogenes Bar Chen pBUN411 50581 Plant BsaI ...

  24. Retrovirus Plasmids

    Type
    Collection
    ...Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral...18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses luciferase Lowe 9044 pMIG...Addgene, including popular packaging, envelope, and transfer plasmids. Viral...scientists discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses...of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description...Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you ...

  25. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...formulated in one of the following three buffers. All buffers are supplemented with 0.001% pluronic F68... or 0.014% Tween 20. Phosphate Buffered Saline (PBS) A universal buffer suitable for any application Balanced...as blood, vitreous, CSF, etc. Not suitable as a buffer for vectors delivered directly to the brain parenchyma...Shannon Boye’s lab at the University of Florida. References for each serotype can be found below under Citations...is available in each of the above serotypes and buffers. Please click on the plasmid name for ordering ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  26. Neurodegeneration Research Collection

    Type
    Collection
    ...cortical neuron differentiation. Held et al. Cell Rep. 2023 Aug 30. Enhance differentiation to mesencephalic...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive disease...are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve cell...of these are due to a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and...al. Nat Methods. 2023 May 4. See More iPSC Differentiation Factors Find plasmids used to transform iPSCs...progression of Huntington’s disease. The foundation offers curated information on tools and reagents (Link...

  27. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored...express both components in the same relative ratio. Different generations of transactivators and promoters are...new window) for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative...Doxycycline, a derivative of tetracycline, is a preferred effector for tet-responsive systems. Dox binds...pSBtet-GP Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al.,...172115 PB-TO-hNGN2 Piggybac Tet-On plasmid for differentiating iPSCs into glutamatergic neurons via human ...NGN2 into AAVS1 safe harbor site, for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...

  28. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2... vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...

  29. Viral Vector Guides and Plasmids

    Type
    Collection
    ...more about the different types of viral vectors and find viral production and transfer plasmids for a ...Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to as just retroviral vectors, have a relatively...Viral Vector Type Each viral vector type exhibits different properties and are suited for specific research...goals. For example, AAV vectors are typically preferred for in vivo studies because of their low immunogenicity...containing the genetic information to be delivered (transfer or shuttle plasmid) and one or more plasmids containing...Specific modifications to these plasmids, such as different promoters or tags, can even direct delivery of...

  30. Rett Syndrome

    Type
    Collection
    ... mutations in methyl-CpG-binding protein 2 confer different severity in Rett syndrome. Neurology . 70,...system for generating Rett syndrome-specific differentiated cells that can be used to understand the mechanisms...immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific... editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...microarray data, ISH image data, and anatomic reference atlases of prenatal and adult human brain (Link...(Link opens in a new window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by ...MeCP2 levels display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol...
Showing: 31 - 60 of 1053 results